Infant formula (IF) constitutes the sole source of mineral intake for infants who are only fed IF. The assurance of the amount of minerals declared on the label and the mineral levels and their chemical forms present a major concern related to providing a good amount of nutrients for absorption by these children. Thus, the objectives of this study were: (i) to evaluate several sample preparation methods for minerals in IF; (ii) to validate an analytical method using an ultrasonic bath for simultaneous determination of the Ca, Cu, Fe, K, Mg, Mn, Na, P and Zn contents in IF by ICP OES and (ii) to establish the optimum analytical conditions of the in vitro method to study the dialyzability of these minerals from IF. The ultrasound-assisted method was shown to conform to ‘green chemistry principles’, being simple, fast and low cost compared with reference methods. The results were similar to those obtained with reference methods (microwave-assisted acid digestion and dry ashing) with regard to selectivity, sensitivity and linearity (r² > 0.999). The accuracy and the precision were verified using certified reference materials, with recoveries and coefficients of variation ranging from 91 to 105% and from 1.1 to 5.2%, respectively. For in vitro dialyzability, the conditions established in this study allowed including an overnight step between the gastric and gastrointestinal stages (accuracy and precision ranging from 81 to 108% and 0.4 to 6.3%, respectively), contributing to establishing an in vitro digestion method suitable for infant gastrointestinal conditions.

中文翻译：

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ICP OES用于婴儿配方食品中矿物质含量和生物可及性研究的简单，快速的超声辅助方法。

婴儿配方食品（IF）是仅接受IF喂养的婴儿摄入矿物质的唯一来源。确保标签上声明的矿物质的含量，矿物质的含量及其化学形式是与这些儿童提供大量营养物质吸收有关的主要问题。因此，本研究的目标是：（i）评估中频矿物的几种样品制备方法；（ii）通过ICP OES验证使用超声波浴同时测定IF中的Ca，Cu，Fe，K，Mg，Mn，Na，P和Zn含量的分析方法，以及（ii）建立最佳分析条件的体外研究中频这些矿物质的透析性的方法。超声辅助方法显示出符合“绿色化学原理”，与参考方法相比简单，快速且成本低。在选择性，灵敏度和线性方面，结果与参考方法（微波辅助酸消解和干灰化）获得的结果相似（r ² > 0.999）。使用认证的参考材料验证了准确性和精密度，其回收率和变异系数分别为91％至105％和1.1％至5.2％。用于体外透析性，在这项研究中建立的条件允许在胃和胃肠道阶段之间进行过夜步骤（准确度和精确度分别为81％至108％和0.4％至6.3％），有助于建立适合婴儿胃肠道的体外消化方法条件。