The sensitive detection of single-cell secreted lactic acid for glycolytic inhibitor screening with a microdroplet biosensor.,Analytical Methods

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The sensitive detection of single-cell secreted lactic acid for glycolytic inhibitor screening with a microdroplet biosensor.
Analytical Methods ( IF 2.7 ) Pub Date : 2020-05-26 , DOI: 10.1039/d0ay00633e
Xuyue Chen ₁ , Rui Shen ₁ , Sidi Liu ₁ , Xiang Xiao ₁ , Jun Yan ₁ , Yiqiu Zhang ₁ , Zhongyun Jiang ₁ , Baoqing Nie ₂ , Jian Liu ₁

Affiliation

Lactic acid (LA) plays an important role in the tumor metabolism and malignant progression of various cancers. Herein, we have developed a one-step, wash-free microfluidic approach with droplet biosensors for the sensitive detection of LA secreted by a single tumor cell. Our assay integrates the enzyme-assisted chemical conversion of LA in small-volume (4.2 nL) droplets for fluorescence signal readout. The microdroplet assay achieved a limit of detection of 1.02 μM and was more sensitive than the commercial ELISA kit by nearly two orders of magnitude. A good specificity has been demonstrated for this assay by testing various ions and biomolecules from the culture medium. This droplet assay allows us to acquire the profiles of the lactic acid secretion of tumor cells under the influence of glycolytic inhibitors at the single-cell level. It offers a useful research tool to study the cell-to-cell differences of LA secretion and glycolytic inhibitor screening for cancer research.

中文翻译：

用微滴生物传感器灵敏检测单细胞分泌的乳酸，以筛选糖酵解抑制剂。

乳酸（LA）在各种癌症的肿瘤代谢和恶性进展中起着重要作用。在本文中，我们已经开发出一种具有液滴生物传感器的一步免洗微流控方法，用于灵敏检测单个肿瘤细胞分泌的LA。我们的测定法将小剂量（4.2 nL）小滴中的LA的酶辅助化学转化过程整合在一起，以读取荧光信号。微滴测定法的检测极限为1.02μM，比市售ELISA试剂盒灵敏近两个数量级。通过测试培养基中的各种离子和生物分子，已证明该测定具有良好的特异性。这种液滴测定使我们能够在单细胞水平上在糖酵解抑制剂的影响下获取肿瘤细胞乳酸分泌的概况。

更新日期：2020-07-02

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