The principle and the techniques applied in DNA extraction play a pivotal role in the obtention of a purified genetic material. The present study investigates the efficiency of eight protocols in the DNA extraction of Hypostomus commersoni, an essential component of South American freshwater ichthyofauna. The quality of samples was assessed through spectrophotometry, gel electrophoresis, and PCR-RAPD amplification. The efficiency of DNA extraction was influenced both by the method applied and the target-tissue of choice. Higher concentrations and yield of DNA were obtained from ocular tissue, with a positive spectrum of incubation in lysis buffer for up to 36 hours after sample collection, using fresh tissues and in the presence of a high concentration of Proteinase K (20 mg.ml-1). In these conditions, samples were successfully amplified. To date, there is no record of description for the parameters analyzed in this work, neither the description of RAPD markers for the species H. commersoni.

中文翻译：

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评价八种Hypostomus commersoni（Valenciennes，1836），Siluriformes：Loricariidae的基因组DNA提取方案。

DNA提取中使用的原理和技术在获得纯化遗传物质中起着关键作用。本研究调查了八种方案在南美洲水鱼鱼的重要组成部分Hyposomus commersoni的DNA提取中的效率。样品的质量通过分光光度法，凝胶电泳和PCR-RAPD扩增进行评估。DNA提取的效率受所用方法和目标组织的选择影响。从眼组织中获得更高的浓度和DNA产量，使用新鲜组织并在高浓度的蛋白酶K（20 mg.ml- 1）。在这些条件下，样品被成功扩增。至今，