The described procedure provides a rapid technique for the detection and semi-quantitation of a large number of drugs in blood. This procedure uses a minimal sample volume and employs a one-step liquid extraction and automated data processing to yield rapid turnaround times. 327 of the most commonly used medicinal and illicit drugs in Australia were selected including various amphetamines, anesthetics, anti-depressants, anti-psychotics, anti-convulsants, benzodiazepines, beta blockers, opioid and non-opioid analgesics, stimulants, THC and a large number of synthetic cannabinoids and other novel psychoactive substances. The extracts were subject to 5-minute chromatography using a Kinetex C18 50 x 4.6 mm 2.6 μm solid-core analytical column and analyzed using a Sciex 3200 Q-TRAP MS-MS (+ ESI, MRM mode, two transitions per analyte). The method was fully validated in accordance with international guidelines. Matrix effects and extraction efficiencies were acceptable with most analytes showing > 80% response and low variation (within 25%RSD). Cannabinoids were most affected by the matrix and yielded poorest recovery values but were still detectable. Precision, accuracy, repeatability and multipoint linearity were assessed for all analytes. The method has been used in routine practice in the forensic toxicology service at the Victorian Institute of Forensic Medicine in over 6000 coronial investigations using both post-mortem and clinical blood specimens. This technique has greatly increased throughput, reduced turnaround times and allowed for rapid same-day analysis of results when needed. The method is routinely used in routine overnight testing with results reported to pathologists within 4 hours of data acquisition. This rapid toxicological technique is used in conjunction with other investigative processes such as full-body CT imaging, review of case circumstances and medical histories to provide an efficient death investigation process.

中文翻译：

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采用自动数据处理的LC-MS-MS高通量检测血液中327种药物1。

所描述的程序提供了一种快速技术，用于检测和半定量血液中的大量药物。该程序使用最小的样品量，并采用一步式液体提取和自动数据处理以快速完成周转时间。澳大利亚选择了327种最常用的药物和非法药物，包括各种苯丙胺，麻醉药，抗抑郁药，抗精神病药，抗惊厥药，苯二氮卓类药物，β受体阻滞剂，阿片类和非阿片类镇痛药，兴奋剂，四氢大麻酚和大型合成大麻素和其他新型精神活性物质的数量。使用Kinetex C18 50 x 4.6 mm 2.6μm固核分析柱对提取物进行5分钟色谱分析，并使用Sciex 3200 Q-TRAP MS-MS（+ ESI，MRM模式，每个分析物两次转换）进行分析。该方法已根据国际准则进行了充分验证。对于大多数分析物，显示出> 80％的响应和较低的变化（在25％RSD之内），基质效应和萃取效率是可以接受的。大麻素受基质影响最大，回收率最差，但仍可检测到。评估了所有分析物的精密度，准确性，可重复性和多点线性。该方法已在维多利亚州法医研究所的法医毒理学服务部门的常规实践中使用了尸体解剖和临床血液标本，进行了6000多次冠状动脉检查。该技术大大提高了通量，缩短了周转时间，并在需要时可以快速进行当天的结果分析。该方法通常用于常规的隔夜测试中，并在数据采集后4小时内将结果报告给病理学家。这种快速的毒理学技术可与其他调查过程（例如全身CT成像，病例情况和病史回顾）结合使用，以提供有效的死亡调查过程。