From our in-house microbial genome database of secondary metabolite producers, we identified a candidate biosynthetic gene cluster for desertomycin from Streptomyces nobilis JCM4274. We report herein the cloning of the 127-kb entire gene cluster for desertomycin biosynthesis using bacterial artificial chromosome vector. The entire biosynthetic gene cluster for desertomycin was introduced in the heterologous host, Streptomyces lividans TK23, with an average yield of more than 130 mg l⁻¹.

中文翻译：

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沙漠霉素生物合成基因簇的鉴定，克隆和异源表达。

从我们内部的次级代谢产物生产者的微生物基因组数据库中，我们鉴定了来自NoStreptomyces nobilis JCM4274的沙漠霉素的候选生物合成基因簇。我们在这里报道了使用细菌人工染色体载体的沙漠霉素生物合成的127kb完整基因簇的克隆。沙漠霉素的整个生物合成基因簇被引入异源宿主链霉菌链霉菌TK23中，平均产量超过130 mg l ^-1。