Oenothera biennis L. (OB), also commonly known as evening primrose, belongs to the Onagraceae family and has the best studied biological activity of all the members in the family. In therapy, the most frequently used type of extracts are from the aerial part, which are the fatty oils obtained from the seeds and have a wide range of medicinal properties. The aim of this study was to evaluate the phytochemical composition and biological activity of OB hydroalcoholic extract and to provide directions for the antimicrobial effect, antiproliferative and pro-apoptotic potential against A375 melanoma cell line, and anti-angiogenic and anti-inflammatory capacity. The main polyphenols and flavonoids identified were gallic acid, caffeic acid, epicatechin, coumaric acid, ferulic acid, rutin and rosmarinic acid. The total phenolic content was 631.496 µgGAE/mL of extract and the antioxidant activity was 7258.67 μmolTrolox/g of extract. The tested extract had a mild bacteriostatic effect on the tested bacterial strains. It was bactericidal only against Candida spp. and S. aureus. In the set of experimental conditions, the OB extract only manifested significant antiproliferative and pro-apoptotic activity against the A375 human melanoma cell line at the highest tested concentration, namely 60 μg/mL. The migration potential of A375 cells was hampered by the OB extract in a concentration-dependent manner. Furthermore, at the highest tested concentration, the OB extract altered the mitochondrial function in vitro, while reducing the angiogenic reaction, hindering compact tumor formation in the chorioallantoic membrane assay. Moreover, the OB extract elicited an anti-inflammatory effect on the experimental animal model of ear inflammation.

中文翻译：

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月见草Biennis L.水醇提取物的植物化学和生物学筛选。

月见草L.（OB），也被称为月见草，属于Onagraceae家族，在该家族中所有成员中生物学活性最高。在治疗中，最常用的提取物类型是地上部分，是从种子中获得的脂肪油，具有广泛的药用特性。这项研究的目的是评估OB水醇提取物的植物化学组成和生物学活性，并为A375黑色素瘤细胞系的抗微生物作用，抗增殖和促凋亡潜力以及抗血管生成和抗炎能力提供指导。鉴定出的主要多酚和类黄酮为没食子酸，咖啡酸，表儿茶素，香豆酸，阿魏酸，芦丁和迷迭香酸。总酚含量为631。496 µgGAE / mL提取物，抗氧化活性为7258.67μmolTrolox/ g提取物。测试的提取物对测试的细菌菌株具有温和的抑菌作用。它仅对念珠菌属有杀菌作用。和金黄色葡萄球菌。在一组实验条件下，OB提取物仅在最高测试浓度（即60μg/ mL）下对A375人黑素瘤细胞系表现出显着的抗增殖和促凋亡活性。OB提取物以浓度依赖的方式阻碍了A375细胞的迁移潜能。此外，在最高测试浓度下，OB提取物在体外改变了线粒体功能，同时减少了血管生成反应，从而阻碍了绒毛膜尿囊膜测定中致密肿瘤的形成。此外，