Dasiphora fruticosa (basionym Potentilla fruticosa) is a shrub, known in traditional medicine for centuries. Due to the wide range of pharmacological effects, interest and applications of D. fruticosa extracts are continually increasing; however, reports on optimization of extraction conditions are scarce. Herein, a multi-step high-pressure extraction process with increasing polarity solvents was developed to isolate valuable fractions from D. fruticosa leaves. Supercritical CO₂ extraction recovered 2.46 g/100 g of lipophilic fraction, rich in polyunsaturated fatty acids. Further, pressurized liquid extractions (PLE) with acetone, ethanol, and water were applied to obtain antioxidant-rich higher polarity extracts. Under optimized PLE conditions, the cumulative polar fraction yield was 29.98 g/100 g. Ethanol fraction showed the highest yield (15.3 g/100 g), TPC values (148.4 mg GAE/g), ABTS^•+, and DPPH^• scavenging capacity (161.1 and 151.8 mg TE/g, respectively). PLE was more efficient than conventional solid–liquid extraction in terms of extraction time, extract yields, and in vitro antioxidant capacity. Phytochemical characterization of PLE extracts by UPLC-Q-TOF-MS revealed the presence of hyperoside, ellagic acid, among other health beneficial phenolic substances. Τhis study highlights the potential of high-pressure extraction techniques to isolate antioxidant-rich fractions from D. fruticosa leaves with multipurpose applications, including the prevention and treatment of chronic diseases.

中文翻译：

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从灌木状五瓣叶（Dasiphora fruticosa L.Rydb。）叶中提取富含抗氧化剂的馏分：工艺优化和提取物表征。

Dasiphora fruticosa（化名Potentilla fruticosa）是一种灌木，在传统医学中已有数百年历史。由于广泛的药​​理作用，金露梅提取物的兴趣和应用正在不断增加。然而，关于提取条件优化的报道很少。在本文中，开发了具有增加极性的溶剂的多步骤高压提取工艺，以从金露梅叶中分离出有价值的馏分。超临界CO ₂萃取可回收到2.46 g / 100 g的亲脂级分，富含多不饱和脂肪酸。此外，用丙酮，乙醇和水进行加压液体萃取（PLE），以获得富含抗氧化剂的高极性萃取物。在优化的PLE条件下，累积的极性馏分收率为29.98 g / 100 g。乙醇级分显示最高产量（15.3 g / 100 g），TPC值（148.4 mg GAE / g），ABTS ^•+和DPPH ^•清除能力（分别为161.1和151.8 mg TE / g）。在萃取时间，萃取物产量和体外抗氧化能力方面，PLE比常规固液萃取更有效。通过UPLC-Q-TOF-MS对PLE提取物进行植物化学表征，揭示了高脂甙，鞣花酸以及其他对健康有益的酚类物质。他的研究强调了高压提取技术具有多种用途，包括从预防和治疗慢性病中分离出金缕梅叶中富含抗氧化剂成分的潜力。