This review summarizes data on the in vitro antimicrobial effectiveness of volatile agents of plant origin and in vitro methods for evaluating their activity in the vapour phase. As a result of literature analysis, the antimicrobial efficacy of vapours from 122 different plant species and 19 pure compounds examined in 61 studies using different in vitro tests against a broad spectrum of microorganisms was identified and summarized. In addition, 11 different techniques found in the literature are described in detail. An original classification of methods based on the solid and liquid matrix volatilization principle is proposed because carrier medium/matrix selection is crucial for the volatilization of any agents tested. This review should be useful for medicinal, pharmaceutical, food, and agricultural experts working in areas related to the management of infectious diseases (especially respiratory and skin infections), food preservation (active packaging), and protection of agriculture products (controlled atmosphere). It may also stimulate the interest of pharmaceutical, cosmetic, food, and agriculture industries in the research and development of new antimicrobial agents of natural origin. Since several original apparatuses previously developed for antimicrobial susceptibility testing in the vapour phase are described in this review, labware manufacturers may also be interested in this topic. The review also provides specific guidelines and recommendations for researchers studying the antimicrobial activity of volatile agents. The article will therefore appeal to communities of industrial stakeholders, pharmacists, physicians, food experts, agriculturists, and researchers in related areas such as pharmacology, medicinal chemistry, microbiology, natural product chemistry, food preservation and plant protection.

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挥发性抗菌剂及其在气相中评估其活性的体外方法：综述

本综述总结了植物来源的挥发性物质的体外抗微生物效力的数据，以及评估它们在气相中的活性的体外方法。作为文献分析的结果，在 61 项研究中使用针对广谱微生物的不同体外测试对 122 种不同植物物种和 19 种纯化合物的蒸气的抗菌功效进行了鉴定和总结。此外，还详细描述了文献中发现的 11 种不同技术。提出了一种基于固体和液体基质挥发原理的原始方法分类，因为载体介质/基质的选择对于任何测试试剂的挥发至关重要。这篇综述应该对医药、制药、食品、以及在传染病管理（尤其是呼吸道和皮肤感染）、食品保存（活性包装）和农产品保护（受控大气）相关领域工作的农业专家。它还可能激发制药、化妆品、食品和农业行业对天然来源的新型抗菌剂的研究和开发的兴趣。由于本综述中描述了先前为气相抗菌药物敏感性测试而开发的几种原始设备，因此实验室器具制造商也可能对这一主题感兴趣。该评论还为研究挥发性物质的抗菌活性的研究人员提供了具体的指导方针和建议。因此，本文将吸引工业利益相关者、药剂师、