Previous studies have demonstrated that polyunsaturated fatty acids (PUFAs) have anti‐inflammatory effects. One specific PUFA, alpha‐linolenic acid (ALA), shows both anti‐inflammatory and antioxidant properties. In the testes, inflammatory mediators are known to increase when orchitis is induced by bacterial lipopolysaccharide (LPS). This study aimed to determine whether the anti‐inflammatory properties of ALA could have a protective effect against LPS‐induced orchitis in mice. The mice were divided into untreated control, orchitis and ALA‐treated orchitis groups. Orchitis was induced by intraperitoneal injection of LPS. The ALA‐treated group was administered ALA by gavage three days before intraperitoneal LPS injection. Cyclooxygenase‐2 (COX‐2), cytosolic phospholipase A2 (cPLA2), inducible nitric oxide synthase (iNOS) enzymes and nuclear factor kappa‐B (NF‐κB) in the testes, as well as serum interleukin 6 (IL‐6) and tumour necrosis factor‐alpha (TNF‐α), were analysed using enzyme‐linked immunosorbent assay (ELISA) tests. LPS administration increased the expression of several inflammatory mediators, including IL‐6, TNF‐α and NF‐κB, as well as the COX‐2, cPLA2 and iNOS enzymes. ALA administration significantly prevented the LPS‐induced increases in these inflammatory mediators and enzymes (p < .05). The anti‐inflammatory and antioxidant effects of ALA may make it a useful candidate for the treatment of orchitis caused by bacterial LPS.

中文翻译：

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α-亚麻酸对脂多糖诱导的小鼠睾丸炎的保护作用。

先前的研究表明，多不饱和脂肪酸（PUFA）具有抗炎作用。一种特定的PUFA，α-亚麻酸（ALA）具有抗炎和抗氧化的特性。在睾丸中，当细菌脂多糖（LPS）诱发睾丸炎时，炎症介质会增加。这项研究旨在确定ALA的抗炎特性是否对小鼠LPS诱发的睾丸炎具有保护作用。将小鼠分为未治疗的对照组，睾丸炎和ALA治疗的睾丸炎组。腹膜内注射LPS可诱发睾丸炎。在腹膜内注射LPS前三天，通过强饲法对ALA治疗组进行了ALA给药。环氧合酶-2（COX-2），胞质磷脂酶A2（cPLA2），使用酶分析了睾丸中的诱导型一氧化氮合酶（iNOS）酶和核因子κB（NF-κB）以及血清白介素6（IL-6）和肿瘤坏死因子-α（TNF-α）联免疫吸附测定（ELISA）测试。LPS给药可增加多种炎症介质的表达，包括IL-6，TNF-α和NF-κB以及COX-2，cPLA2和iNOS酶。ALA给药可显着阻止LPS诱导的这些炎症介质和酶的增加（p  <.05）。ALA的抗炎和抗氧化作用可能使其成为治疗由细菌LPS引起的睾丸炎的有用候选药物。