Hepatoprotective peptides purified from Corbicula fluminea and its effect against ethanol‐induced LO2 cells injury,International Journal of Food Science & Technology

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Hepatoprotective peptides purified from Corbicula fluminea and its effect against ethanol‐induced LO2 cells injury
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-05-26 , DOI: 10.1111/ijfs.14649
Jiaoyan Ren _{1,

2} , Wanqian Sha ₁ , Shuaiming Shang ₁ , Erdong Yuan ₁

Affiliation

This study was focused on the purification, identification and mechanism of hepatoprotective peptides from Corbicula fluminea by using Sephadex G‐15 chromatography, UPLC–MS/MS, oxygen radical absorbance capacity (ORAC) assay, cell experiment and molecular docking. Six identified peptides were synthesised chemically and subjected to evaluate hepatoprotective effect. ORAC value and hepaprotective effect against ethanol‐induced LO2 cell injury in vitro were determined. The results showed that Tyr‐Phe‐Leu‐Pro (YP‐4) and Leu‐Val‐Tyr‐Pro (LP‐4) exhibited the strongest antioxidant activity and significantly increased the viability of ethanol‐injured LO2 cells. These two peptides significantly reduced ROS levels and efficiently inhibited the decrease of mitochondrial membrane potential in ethanol‐injured LO2 cells. Molecular docking revealed that YP‐4 and LP‐4 possess potential inhibitory activity on CYP2E1 and thus reduce ethanol‐induced oxidative stress. It is suggested that YP‐4 and LP‐4 have good hepatoprotective effect against alcoholic liver injury.

中文翻译：

从草提取的保肝肽及其对乙醇诱导的LO2细胞损伤的作用

这项研究的重点是通过使用Sephadex G-15色谱法，UPLC-MS / MS，氧自由基吸收能力（ORAC）测定，细胞实验和分子对接，来从黄Cor中的肝保护肽纯化，鉴定和机理。化学合成了六个鉴定出的肽，并进行了肝保护作用的评估。ORAC值和hepaprotective效果对乙醇诱导的LO2细胞损伤的体外被确定。结果表明，Tyr-Phe-Leu-Pro（YP-4）和Leu-Val-Tyr-Pro（LP-4）表现出最强的抗氧化活性，并显着提高了乙醇损伤的LO2细胞的活力。这两种肽显着降低了ROS的水平，并有效抑制了乙醇损伤的LO2细胞中线粒体膜电位的降低。分子对接显示YP-4和LP-4具有对CYP2E1的潜在抑制活性，从而降低了乙醇诱导的氧化应激。建议YP-4和LP-4对酒精性肝损伤具有良好的肝保护作用。

更新日期：2020-05-26

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