Introduction

The objective of this study is to evaluate the effect of basic fibroblast growth factor (bFGF) on the proliferation and secretion activity of feline adipose-derived mesenchymal stem cells (MSC).

Methods

Feline MSC isolated from the subcutaneous adipose tissue of cats were cultured with or without bFGF.

Results

The bFGF addition enhanced the proliferation of feline MSC to a significant great extent compared with that without bFGF, although the cell proliferation tended to increase with the bFGF concentration. In addition, adipogenic and osteogenic staining assay demonstrated that the bFGF addition allowed MSC to maintain the differentiation ability even after the proliferation. Moreover, no change in the surface markers of MSC was observed between the cultures with or without bFGF. A quantitative RT-PCR assay revealed that the HGF and TSG-6 expression significantly increased by the bFGF addition. The highest mRNA expression of MMP-2 was observed for cells cultured in 1000 ng/ml bFGF concentration.

Conclusions

The culture with bFGF is a promising way to enhance the proliferation, and HGF secretion ability of MSC as well as maintain their differentiation ability and immunophenotype nature.

中文翻译：

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碱性成纤维细胞生长因子增强猫间充质干细胞的增殖和肝细胞生长因子表达。

介绍

本研究的目的是评估碱性成纤维细胞生长因子 (bFGF) 对猫脂肪来源间充质干细胞 (MSC) 增殖和分泌活性的影响。

方法

从猫的皮下脂肪组织中分离的猫 MSC 在有或没有 bFGF 的情况下进行培养。

结果

与未添加 bFGF 相比，添加 bFGF 显着增强了猫 MSC 的增殖，尽管细胞增殖随着 bFGF 浓度的增加而增加。此外，脂肪生成和成骨染色测定表明，添加 bFGF 使 MSC 即使在增殖后也能保持分化能力。此外，在有或没有 bFGF 的培养物之间没有观察到 MSC 表面标志物的变化。定量 RT-PCR 分析表明，添加 bFGF 后 HGF 和 TSG-6 表达显着增加。对于在 1000 ng/ml bFGF 浓度下培养的细胞，观察到 MMP-2 的最高 mRNA 表达。

结论

bFGF 培养是增强 MSC 增殖和 HGF 分泌能力以及保持其分化能力和免疫表型性质的有前景的方法。