Abstract The beneficial biological properties of l -fucose have extended its commercial application potential in pharmaceutical, cosmetic, and food industries. Enzymatic production of l -fucose with l -fucose isomerase ( l -FucI) is considered a selective, green, and efficient strategy. Efficient sugar production requires thermophilic enzymes with increased reaction rate, reduced risk of microbial contamination, and high sugar solubility. No study has evaluated the applicability of thermophilic l -FucI for l -fucose production. In this study, we explored the biochemical properties of a thermostable l -FucI from Thermanaeromonas toyohensis (TtFucI) using l -fuculose as a substrate. The recombinant TtFucI exhibited thermophilicity and optimum activity at 70 °C. The specific activity, Km, and kcat toward l -fuculose were 199.8 U/mg, 33.4 mM, and 901.7 s−1, respectively. Mn2+ ions increased the activity of the enzyme by ∼10 times and enhanced its thermal stability. Our study, on l -fucose synthesis by thermostable l -FucI, suggests the potential application of this enzyme for the industrial production of l -fucose.

中文翻译：

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来自丰水产热单胞菌的嗜热 l-岩藻糖异构酶，用于从 l-岩藻糖合成 l-岩藻糖

摘要 l-岩藻糖的有益生物学特性扩展了其在制药、化妆品和食品行业的商业应用潜力。使用 l -岩藻糖异构酶 (l -FucI) 酶促生产 l -岩藻糖被认为是一种选择性、绿色和有效的策略。高效的糖生产需要具有更高反应速率、更低微生物污染风险和高糖溶解度的嗜热酶。没有研究评估嗜热 l -FucI 对 l -岩藻糖生产的适用性。在这项研究中，我们使用 l -岩藻糖作为底物，探索了来自丰隆产热单胞菌 (TtFucI) 的热稳定 l -FucI 的生化特性。重组 TtFucI 在 70 °C 时表现出嗜热性和最佳活性。对 l-岩藻糖的比活性、Km 和 kcat 为 199.8 U/mg、33.4 mM 和 901。分别为 7 s−1。Mn2+ 离子使酶的活性提高了约 10 倍，并增强了其热稳定性。我们关于通过热稳定 l -FucI 合成 l -岩藻糖的研究表明该酶在 l -岩藻糖的工业生产中的潜在应用。