Cystic echinococcosis (CE) is a zoonotic disease caused by the cestode parasite Echinococcus granulosus. The disease has an important impact on human health as well as economic costs including the cost of treatment as well as loss of productivity for the livestock industry. In many parts of the world where the disease is endemic, sheep and other livestock play an important role in the parasite's transmission. A vaccine to protect livestock against CE can be effective in reducing transmission and economic costs of the disease. A recombinant antigen vaccine has been developed against infection with E. granulosus (EG95) which could potentially be used to reduce the level of E. granulosus transmission and decrease the incidence of human infections. Further development of the EG95 recombinant vaccine as a combined product with clostridial vaccine antigens is one potential strategy which could improve application of the hydatid vaccine by providing an indirect economic incentive to livestock owners to vaccinate against CE. In this study we investigated the efficacy of the EG95 recombinant vaccine produced in Morocco by vaccination of sheep, including a combined vaccine incorporating EG95 and clostridia antigens. Vaccination with EG95 either as a monovalent vaccine or combined with clostridia antigens, protected sheep against a challenge infection with E. granulosus eggs and induced a strong, long lasting, and specific antibody response against the EG95 antigen.

囊性棘球co虫病（CE）是一种由site虫寄生棘粒棘球E球菌引起的人畜共患疾病。该疾病对人类健康以及包括治疗费用在内的经济成本以及畜牧业生产力的下降都具有重要影响。在世界上许多地方流行的地方，绵羊和其他牲畜在寄生虫的传播中起着重要作用。保护牲畜免受CE侵害的疫苗可以有效减少该疾病的传播和经济成本。已经开发了针对颗粒大肠杆菌（EG95）感染的重组抗原疫苗，可用于降低颗粒大肠杆菌的水平传播并减少人类感染的发生率。EG95重组疫苗与梭菌疫苗抗原的联合产品的进一步开发是一种潜在的策略，可通过向畜牧业者提供间接经济诱因来接种CE疫苗，从而改善hydatid疫苗的应用。在这项研究中，我们调查了在摩洛哥通过羊接种而生产的EG95重组疫苗的功效，包括结合了EG95和梭菌抗原的联合疫苗。以单价疫苗或与梭状芽孢杆菌抗原组合的形式接种EG95疫苗可以保护绵羊免受颗粒大肠杆菌的卵的挑战性感染，并诱导针对EG95抗原的强烈，持久且特异性的抗体反应。