Herein we demonstrate cultivation-dependent siderophore production by the actinomycete Gordonia rubripertincta CWB2. The strain produces mostly citrate, but also desferrioxamine E (DFOE) and new hydroxamate-type siderophores. The production of hydroxamate-like siderophores is influenced by cultivation conditions, for example available carbon sources or presence of metals, such as the rare earth erbium or the heavy metal lead. By cultivation with succinate and extraction with an adsorbing resin (XAD) we purified the G. rubripertincta CWB2 siderophores (yield up to 178 mg L⁻¹). The respective workflow comprises genome mining, cultivation, and overproduction strategies, a rapid screening procedure, as well as traditional structure enrichment and structure elucidation methods. This combination of methods allows the discovery of new natural products with metal complexation capacity, also for lanthanides of commercial value. G. rubripertincta CWB2 carries a desferrioxamine-like biosynthetic gene cluster. Its transcription was proven by a transcriptomic approach comparing expression levels of the selected gene cluster during cultivation in iron-depleted and repleted media. Further investigation of the siderophores of this desferrioxamine producing Actinobacterium could lead to new structures.

在这里，我们展示了放线菌戈登尼亚红rippleincincta CWB2的依赖培养的铁载体生产。该菌株主要产生柠檬酸盐，但也会产生去铁草胺E（DFOE）和新的异羟肟酸酯型铁载体。异羟肟酸酯样铁载体的产生受培养条件的影响，例如可利用的碳源或金属的存在，例如稀土或重金属铅。通过用琥珀酸酯培养并用吸附树脂（XAD）萃取，我们纯化了rubripertincta CWB2铁载体（产量高达178 mg L ^-1）。各自的工作流程包括基因组挖掘，培养和过量生产策略，快速筛选程序以及传统的结构富集和结构阐明方法。方法的这种组合允许发现具有金属络合能力的新天然产物，也具有商业价值的镧系元素。G. rubripertincta CWB2带有类似去铁草胺的生物合成基因簇。通过转录组学方法证明了其转录，该方法比较了在贫铁和富铁培养基中培养期间所选基因簇的表达水平。对该产去铁草胺的放线菌的铁载体的进一步研究可能导致新的结构。