Faithful analysis of transcripts represents a critical component of the gene discovery, genomic characterization and annotation process in species without a reference genome. The present study generated 28,001 full-length transcripts of Misgurnus anguillicaudatus using the Pacific Bioscience (PacBio) single-molecule real-time (SMRT) sequencing system. A total of 77,346 consensus isoforms were identified, and 18,991 complete open reading frames (ORFs) detected. In total, 1345 lncRNAs were identified with high-confidence, with additional identification of a number of well-known transcription factors. The present study may facilitate additional exploration of the genomic signatures of M. anguillicaudatus.

对转录本的忠实分析代表了没有参考基因组的物种中基因发现，基因组表征和注释过程的关键组成部分。本研究使用太平洋生物科学（PacBio）单分子实时（SMRT）测序系统生成了28,001条鳗Mi（Misgurnus anguillicaudatus）全长转录本。总共鉴定出77,346个共有同工型，并检测到18,991个完整的开放阅读框（ORF）。总共鉴定出1345个lncRNA，并额外鉴定了许多众所周知的转录因子。本研究可能有助于进一步研究鳗螺的基因组特征。