Polycyclic musk compounds have been identified in environmental matrices (water, sediment and air) and in biological tissues in the last decade, yet only minimal attention has been paid to their chronic toxicity in the marine environment. In the present research, the clams Ruditapes philippinarum were exposed to 0.005, 0.05, 0.5, 5 and 50 μg/L of the fragrances Galaxolide® (HHCB) and Tonalide® (AHTN) for 21 days. A battery of biomarkers related with xenobiotics biotransformation (EROD and GST), oxidative stress (GPx, GR and LPO) and genotoxicity (DNA damage) were measured in digestive gland tissues. HHCB and AHTN significantly (p < 0.05) induced EROD and GST enzymatic activities at environmental concentrations. Both fragrances also induced GPx activity. All concentrations of both compounds induced an increase of LPO and DNA damage on day 21. Although these substances have been reported as not acutely toxic, this study shows that they might induce oxidative stress and genotoxicity in marine organisms.

在过去的十年中，已经在环境基质（水，沉积物和空气）和生物组织中发现了多环麝香化合物，但是对于它们在海洋环境中的慢性毒性只有很少的关注。在本研究中，蛤仔菲律宾蛤仔将其暴露于0.005、0.05、0.5、5和50μg/ L的香料（HHCB）和Tonalide®（AHTN）中21天。在消化腺组织中测量了一系列与异生物素生物转化（EROD和GST），氧化应激（GPx，GR和LPO）和遗传毒性（DNA损伤）相关的生物标记。HHCB和AHTN在环境浓度下显着（p <0.05）诱导EROD和GST的酶活性。两种香精也诱导GPx活性。两种化合物的所有浓度都在第21天引起LPO和DNA损伤的增加。尽管据报道这些物质没有剧毒，但这项研究表明，它们可能在海洋生物中引起氧化应激和遗传毒性。