Journal of Advanced Research ( IF 11.4 ) Pub Date : 2020-05-25 , DOI: 10.1016/j.jare.2020.05.018 Hany Ibrahim 1 , Ahmed Serag 2 , Mohamed A Farag 3, 4
|
Background
Hydrogen sulfide (H2S) is currently considered among the endogenously produced gaseous molecules that exert various signaling effects in mammalian species. It is the third physiological gasotransmitter discovered so far after NO and CO. H2S was originally ranked among the toxic gases at elevated levels to humans. Currently, it is well-known that, in the cardiovascular system, H2S exerts several cardioprotective effects including vasodilation, antioxidant regulation, inhibition of inflammation, and activation of anti-apoptosis. With an increasing interest in monitoring H2S, the development of analysis methods should now follow.
Aim of review
This review stages special emphasis on the several analytical technologies used for its determination including spectroscopic, chromatographic, and electrochemical methods. Advantages and limitations with regards to the application of each technique are highlighted with special emphasis on its employment for H2S in vivo measurement i.e., biofluids, tissues.
Key Scientific Concepts and important findings of Review
Fluorescence methods applied for H2S measurement offer an attractive non-invasive and promising approach in addition to its selectivity, however they cannot be considered as H2S-specific probes. On the other hand, colorimetric assays are among the most common methods used for in vitro H2S detection, albeit their employment in vivo H2S measurement has not yet been possible . Separation techniques such as gas or liquid chromatography offer higher selectivity compared to direct spectrophotometric or fluorescence methods especially for suitable for endpoint H2S measurements i.e. plasma or tissue samples. Despite all the developed analytical procedures used for H2S determination, the need for highly selective, much work should be devoted to resolve all the pitfalls of the current methods.
中文翻译：
第三种气体递质H2S检测的新兴分析工具全面综述
背景
硫化氢 (H 2 S) 目前被认为是内源产生的气体分子之一，可在哺乳动物物种中发挥各种信号作用。它是继NO和CO之后迄今为止发现的第三种生理气体递质。H 2 S最初被列为对人类含量较高的有毒气体之一。目前，众所周知，H 2 S在心血管系统中发挥多种心脏保护作用，包括血管舒张、抗氧化调节、抑制炎症、激活抗凋亡等。随着人们对监测 H 2 S 的兴趣日益浓厚，分析方法的发展也随之而来。
审查目的
本综述特别强调用于测定的几种分析技术，包括光谱、色谱和电化学方法。强调了关于每种技术的应用的优点和局限性，特别强调其用于H 2 S体内测量（即生物流体、组织）的应用。
关键科学概念和评论的重要发现
用于H 2 S测量的荧光方法除了具有选择性之外，还提供了一种有吸引力的非侵入性且有前景的方法，但它们不能被视为H 2 S特异性探针。另一方面，比色测定法是体外H 2 S 检测最常用的方法之一，尽管它们尚未可能用于体内H 2 S 测量。与直接分光光度法或荧光法相比，气相色谱或液相色谱等分离技术提供了更高的选择性，尤其适用于终点 H 2 S 测量，即。血浆或组织样本。尽管所有已开发的分析程序都用于H 2 S 测定，但仍需要高度选择性、大量工作来解决当前方法的所有缺陷。
京公网安备 11010802027423号