Meiotic entry and progression require dynamic regulation of germline gene expression. m⁶A on mRNAs and recognition by YTHDC2 has been known as post-transcriptional regulatory complex, but the roles of this regulator remain unclear for meiotic initiation and progression in female germ cells (FGCs). This study showed that m⁶A modification occurred mainly in FGCs rather than ovarian somatic cells (SOMAs), and m⁶A levels in FGCs increased significantly with meiotic initiation. m⁶A inhibition suppressed expression of the meiotic markers and affected the percent of FGCs at zygotene, pachytene and diplotene stage respectively. YTHDC2 expression also increased in the same pattern with m⁶A. Ythdc2 knockdown decreased the percent of STRA8-positive FGCs and altered the percent of FGCs at zygotene and pachytene stage respectively. Taken together, these results suggest that mRNA m⁶A modification and YTHDC2 expression are essential for meiotic initiation and progression in FGCs.

减数分裂进入和进展需要动态调节种系基因表达。mRNA 上的m ⁶ A 和 YTHDC2 的识别被称为转录后调节复合物，但该调节器在雌性生殖细胞 (FGC) 中的减数分裂起始和进展中的作用仍不清楚。该研究表明，m ⁶ A 修饰主要发生在 FGC 而非卵巢体细胞 (SOMA) 中，并且FGC 中的m ⁶ A 水平随着减数分裂的开始而显着增加。m ⁶抑制抑制了减数分裂标志物的表达并分别影响了合子期、粗线期和二线期的 FGC 百分比。YTHDC2 表达也以与 m ⁶ A相同的模式增加。Ythdc2敲低降低了 STRA8 阳性 FGC 的百分比，并分别改变了合子和粗线期 FGC 的百分比。总之，这些结果表明 mRNA m ⁶ A 修饰和 YTHDC2 表达对于 FGC 的减数分裂起始和进展至关重要。