Abstract Proteins in the common legume Macrotyloma uniflorum have inherent antimicrobial properties and can be converted into films for food, medical and other applications. Crude proteins were extracted from horsegram seeds and were converted into thin films by casting approach. Amino acid analysis of the extracted protein confirmed the presence of both essential and non- essential amino acids required for food applications. However, the protein films have relatively weak mechanical properties and poor aqueous stability which can be substantially improved by crosslinking with a biocompatible crosslinker, catechol. Crosslinked films had strength of 7.32 MPa and were stable in water and cell culture media for upto 4 days suggesting their suitability for tissue engineering and other medical applications. The swelling % of the films was directly proportional to the amount of catechol used. The 20% catechol crosslinked films displayed significant antibacterial activity against Bacillus cereus and E. coli in comparison to the uncrosslinked films. In addition to demonstrating good antifungal activity against Aspergillus niger, the films were conducive to the growth of L929 mouse fibroblast cells, better than Zn @20 ppm. Overall, large availability, presence of essential amino acids, ability to develop films and other biomaterials with desired properties and cytocompatibility make horsegram proteins desirable for food, medical and other applications.

中文翻译：

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儿茶酚交联马格蛋白膜的生物相容性的开发、表征和评价

摘要 常见豆科植物 Macrotyloma uniflorum 中的蛋白质具有固有的抗菌特性，可转化为用于食品、医疗和其他应用的薄膜。从马荠种子中提取粗蛋白质，并通过浇铸方法将其转化为薄膜。提取的蛋白质的氨基酸分析证实了食品应用所需的必需和非必需氨基酸的存在。然而，蛋白质膜具有相对较弱的机械性能和较差的水稳定性，这可以通过与生物相容性交联剂儿茶酚交联得到显着改善。交联膜的强度为 7.32 MPa，在水和细胞培养基中稳定长达 4 天，表明它们适用于组织工程和其他医学应用。薄膜的溶胀百分比与儿茶酚的用量成正比。与未交联的薄膜相比，20% 儿茶酚交联的薄膜对蜡样芽孢杆菌和大肠杆菌表现出显着的抗菌活性。除了对黑曲霉表现出良好的抗真菌活性外，这些薄膜有利于 L929 小鼠成纤维细胞的生长，优于 Zn @20 ppm。总的来说，大量的可用性、必需氨基酸的存在、开发具有所需特性和细胞相容性的薄膜和其他生物材料的能力使马匹蛋白成为食品、医疗和其他应用的理想选择。除了对黑曲霉表现出良好的抗真菌活性外，这些薄膜有利于 L929 小鼠成纤维细胞的生长，优于 Zn @20 ppm。总的来说，大量的可用性、必需氨基酸的存在、开发具有所需特性和细胞相容性的薄膜和其他生物材料的能力使马匹蛋白成为食品、医疗和其他应用的理想选择。除了对黑曲霉表现出良好的抗真菌活性外，这些薄膜有利于 L929 小鼠成纤维细胞的生长，优于 Zn @20 ppm。总的来说，大量的可用性、必需氨基酸的存在、开发具有所需特性和细胞相容性的薄膜和其他生物材料的能力使马匹蛋白成为食品、医疗和其他应用的理想选择。