The distribution of viruses and their impact on prokaryotes were studied using the epifluorescence and transmission electron microscopy along a transect, located at 130°E from the area adjacent to the Lena River delta next to the shelf area, the continental slope area and the deep-sea regions of the Laptev Sea in September 2015. The abundance of planktonic prokaryotes (N_P) and free viral particles (N_V), the frequency of visibly virus-infected prokaryotic cells (FVIC), and the viral-mediated prokaryotic mortality (VMP) varied within the following ranges: (0.1–3.1) × 10⁶ cells mL⁻¹, (0.1–4.4) × 10⁶ viruses mL⁻¹, 0.3–1.7% of N_P, and 2.1–12.2% of the total mortality of prokaryotes, respectively, and reached maximum values at the inner shelf area. The percentage of viruses attached to prokaryotic cells varied from 2 to 6% of N_V in the surface waters of the outer shelf and the deep-sea areas to 43–46% at the depth of 100–140 m in the continental slope area. The percentage of viruses that were attached to detrital or mineral microparticles varied from less than 1% in the waters of the deep-sea area to 27–50% in the waters of the inner shelf area adjacent to the Lena River delta. A negative correlation was found between the fraction of lysogenic prokaryotes and the FVIC values (r = – 0.60, p < 0.05).

In the 20-cm bottom water layer above the sediment the N_P, N_V, FVIC and VMP values were (0.8–3.0) × 10⁶ cells mL⁻¹, (1.6–3.4) × 10⁶ viruses mL⁻¹, 0.5–1.2%, and 3.7–9.5%, respectively. In the surface 2-cm layer of the sediment these parameters were (1.8–4.4) × 10⁹ cells cm⁻³, (0.8–2.3) × 10⁹ viruses cm⁻³, 0.2–1.0%, and 1.4–7.8%, respectively. In the bottom water layer the percentage of viral particles attached to prokaryotes and to detrital and mineral particles was 10–29% and 20–79% of N_V, respectively. In the surface sediments the percentage of viral particles attached to prokaryotes was considerably higher compared to the bottom water layer and ranged from 44 to 57% of N_V. The capsid size of the viral particles from the pelagic zone, bottom water layer and surface sediments was on average 71 ± 14, 74 ± 7 and 63 ± 7 nm, respectively. In all these habitats, viruses from the size class of 60–100 nm prevailed.

The relatively high abundance of prokaryotes and viruses, as well as the high FVIC values in the freshwater-seawater mixing zone (the highest values were recorded in the waters with a salinity of 9.6 psu) were apparently caused by the influx of considerable amounts of nutrients, and dissolved and particulate organic matter that come with the river runoff. At the same time, the high concentration of small detrital and mineral particles in the freshwaters lead to considerable adsorption of viruses by these particles and caused a decrease in the FVIC in the coastal areas adjacent to the Lena River delta.

使用落射荧光和透射电子显微镜沿着一条样带研究了病毒的分布及其对原核生物的影响，该样带位于距Lena河三角洲相邻区域，毗邻陆架区域，大陆斜坡区域和深海的130°E区。 2015年9月在拉普捷夫海的海域。浮游生物原核生物（N _P）和游离病毒颗粒（N _V）的含量，可见病毒感染的原核细胞的频率（FVIC）和病毒介导的原核生物死亡率（VMP）在以下范围内变化：（0.1–3.1）×10 ^6个 细胞mL ^-1，（0.1–4.4）×10 ^6个病毒mL ^-1，0.3-1.7的％Ñ _P，和原核生物中，分别的总死亡率的2.1-12.2％，以及在内部搁板面积达到最大值。附着在原核细胞上的病毒的百分比从外层架子和深海区域的地表水的N _V的2％到6％，到大陆斜坡区域100-140 m深度的43-46％。附着在碎屑或矿物微粒上的病毒所占百分比从深海区域的不到1％到与Lena河三角洲相邻的内陆架区域的不到27％至50％不等。发现溶原性原核生物的分数与FVIC值呈负相关（r  = – 0.60，p <0.05）。

在沉积物上述20厘米底部水层Ñ _P，Ñ _V，FVIC和VMP值分别为（0.8-3.0）×10个⁶ 细胞毫升^-1，（1.6-3.4）×10种⁶病毒毫升^-1，0.5分别为–1.2％和3.7–9.5％。在沉积物这些参数分别为（1.8-4.4）×10的表面为2cm层⁹ 细胞厘米^-3，（0.8-2.3）×10 ⁹病毒厘米^-3，0.2-1.0％，和1.4-7.8％，分别。在底部水层中，附着于原核生物以及碎屑和矿物质颗粒的病毒颗粒的百分比为氮的10–29％和20–79％_V分别。在表层沉积物中，附着在原核生物上的病毒颗粒的百分比比底部水层高得多，范围为N _V的44％至57％。来自上层带，底水层和表面沉积物的病毒颗粒的衣壳大小平均分别为71±14、74±7和63±7 nm。在所有这些栖息地中，都盛行了60-100 nm大小的病毒。

显然，大量营养物质的流入是由于原核生物和病毒相对丰富，以及淡水-海水混合区的FVIC值较高（在盐度为9.6 psu的水中记录的最高值）。以及河流径流带来的溶解和颗粒状有机物。同时，淡水中的少量碎屑和矿物颗粒高浓度导致这些颗粒对病毒的大量吸附，并导致与莉娜河三角洲相邻的沿海地区的FVIC下降。