The burrowing crab Neohelice granulata is a key omnivorous species in intertidal areas along the southwestern Atlantic from southern Brazil to northern Argentinean Patagonia. This crab is adapted to starvation and can endure natural periods of food deprivation. The metabolic adjustments during starvation depend on the type of diet the crabs were fed previously. Since eyestalk-crustacean hyperglycemic hormone (CHH) is the principal regulator of glucose homeostasis in decapods, we investigated whether CHH transcription was affected by diet composition and starvation. Crabs were maintained in the laboratory for two weeks and subsequently divided in two groups. One received a high carbohydrate (HC) diet, and the other was fed a high protein (HP) diet. After this period, they were starved for four weeks. The full-length cDNA sequence of N. granulata CHH was determined and aligned with CHH sequences of other crabs. Levels of circulating glucose and glycogen were higher in the hepatopancreas and muscle of the HC-fed group and decreased after starvation. Glucose and glycogen concentrations were not altered by starvation in the HP group. Triglyceride levels within the hemolymph were not altered by diet or starvation. However, triglycerides concentration was higher in the hepatopancreas of HC compared to HP-fed group. Long-term starvation and diet composition did not affect CHH transcription.

穴居蟹Neohelice granulata是从巴西南部到阿根廷北部巴塔哥尼亚西南大西洋沿潮间带的主要杂食性物种。这种螃蟹适合饥饿，可以忍受自然时期的食物匮乏。饥饿期间的代谢调节取决于先前喂养螃蟹的饮食类型。由于眼柄-甲壳类高血糖激素（CHH）是十足动物体内葡萄糖稳态的主要调节剂，因此我们研究了CHH转录是否受饮食组成和饥饿影响。将螃蟹在实验室中放置两个星期，然后分为两组。一个人接受高碳水化合物（HC）饮食，另一个接受高蛋白（HP）饮食。在此之后，他们饿了四个星期。颗粒猪笼草的全长cDNA序列确定CHH并与其他蟹的CHH序列比对。HC喂养组的肝胰脏和肌肉中循环葡萄糖和糖原的水平较高，饥饿后下降。在HP组中，饥饿不会改变葡萄糖和糖原的浓度。饮食或饥饿不会改变血淋巴中的甘油三酸酯水平。但是，与HP喂养组相比，HC肝胰腺中的甘油三酸酯浓度更高。长期饥饿和饮食组成不会影响CHH的转录。