Frass, a by-product of the larval meal industry, is heterogeneous and includes larval excrement, exoskeleton sheds and residual feed ingredients along with abundant nutrients, chitin and beneficial microbes. The present study was performed to evaluate the changes in growth, feed utilization, body composition, hematology, serum chemistry, immune responses and disease resistance of hybrid tilapia, Nile x Mozambique (Oreocromis niloticus x O. mozambique) fed diets containing frass from black soldier fly larvae, Hermetia illucens. Five diets containing frass at levels of 0, 5, 10, 20, and 30% as partial replacements of a combination of soybean meal, wheat short and corn meal on an equal protein basis were fed to juvenile hybrid tilapia (2.6 ± 0.035 g) in quadruplicate aquaria to apparent satiation twice a day for the first two weeks and once daily for rest of the feeding trail. Final weight gain was significantly increased in fish fed the diet including the highest level of frass (30%). Fish fed diets containing frass (5% to 30%) had significantly higher protein efficiency than the group fed diet without frass (control diet). Feed intake and feed utilization efficiency were not significantly affected by dietary treatments. Survival during the feeding trail, whole-body composition, hematological parameters, and serum biochemistry were not affected by dietary treatment. Serum complement activity of fish fed 30% dietary frass was significantly higher than that of fish fed other treatments. Fish fed the diets containing frass showed significant dose-dependent trends in survival against both Flavobacterium columnare and Streptococcus iniae challenges. Frass from the larvae of black solder flies fed Distillers’ dried grains with solubles has potential for use as feed ingredient for improving growth of hybrid tilapia. Use of frass in tilapia diets may prove beneficial by improving innate immune components and the resistance of hybrid tilapia against bacterial infection.

Frass是幼虫行业的副产品，种类繁多，包括幼虫排泄物，外骨骼棚和残留的饲料成分以及丰富的营养素，甲壳质和有益微生物。进行本研究以评估杂交罗非鱼，尼罗河莫桑比克（莫桑比克Oreocromis niloticus x O.莫桑比克）饲喂含黑士兵解酒的日粮的生长，饲料利用率，身体组成，血液学，血清化学，免疫反应和抗病性的变化。蝇幼虫，Hermetia illucens。将五种日粮含量分别为0、5、10、20和30％的菜籽粉替代以相同蛋白质为基础的豆粕，小麦短粉和玉米粉的组合的替代饲料（2.6±0.035 g）在前两个星期中，将水族箱一式四份地养成明显的饱足感，其余的喂食轨迹则每天一次。饲喂日粮中最高水平的鱼（30％）后，最终体重增加显着增加。以鱼为食（含5％至30％）的日粮比不以鱼为食的组（对照日粮）具有更高的蛋白质效率。饮食处理对饲料摄入和饲料利用率没有显着影响。饮食治疗不会影响喂养过程中的存活率，全身组成，血液学参数和血清生化指标。饲喂30％日粮的鱼的血清补体活性显着高于饲喂其他处理的鱼。饲喂含苦菜的鱼对两种鱼的存活率均表现出明显的剂量依赖性趋势柱状黄杆菌和猪链球菌的挑战。用黑锡蝇幼虫的幼虫喂食Distillers的干粮中掺有可溶物，有潜力用作饲料原料，以改善杂交罗非鱼的生长。通过改善先天免疫成分和杂交罗非鱼对细菌感染的抵抗力，在罗非鱼日粮中使用草甘膦可能被证明是有益的。