Methicillin-resistant Staphylococcus aureus (MRSA), an important widespread cause of severe infection in both humans and animals, is a significant pathogen of public health concern. This study examined the presence of MRSA in 400 samples comprising 200 raw milks (145 from goat and 55 from sheep) and 200 nasal swabs (145 from goats and 55 from sheep) collected from ten different locations in Abeokuta, Nigeria. Samples were examined using standard bacteriological methods for the isolation and identification of Staphylococcus aureus and culture on oxacillin (6 μg/ml) and cefoxitin (2 μg/ml) selective media for the detection of MRSA. Suspected MRSA isolates were confirmed by latex agglutination test for the detection of penicillin-binding protein 2a (PBP2a). Antibiotic susceptibility testing was determined by Kirby Bauer disc diffusion method. Staphylococcus aureus was detected in 72 (18%) of 400 samples of which 52 (13%) were confirmed as MRSA. Methicillin-resistant S. aureus was detected in raw milk (37 of 200; 18.5%) and nasal swab (15 of 200; 7.5%). There was no significance difference (p > 0.05) in the prevalence of MRSA in sheep (37.7%) and goat (23.4%). The MRSA isolates showed resistance to ampicillin (100%), cloxacillin (100%), sulphamethoxazole-trimethoprim (100%), amoxicillin-clavulanate (84.6%), ceftriaxone (75%), cefuroxime (69.2%), erythromycin (65.4%), streptomycin (38.5%), ciprofloxacin (23.1%), pefloxacin (21.2%) and gentamicin (17.3%). The presence of multidrug-resistant MRSA in small ruminants reared in Abeokuta metropolis may be due to regular use of antibiotics and unhygienic practices by farmers. This in turn constitutes a potential public health risk to the owners, consumers of small ruminant products and the general populace.

耐甲氧西林金黄色葡萄球菌（MRSA）是人类和动物严重感染的重要广泛原因，是引起公众健康的重要病原体。这项研究检查了从尼日利亚十个不同地点收集的400份样品中是否存在MRSA，这些样品包括200份原奶（145份来自山羊，55份来自绵羊）和200支鼻拭子（145份来自山羊，55份来自绵羊）。使用标准细菌学方法检查样品，以分离和鉴定金黄色葡萄球菌并在奥沙西林（6μg/ ml）和头孢西丁（2μg/ ml）选择性培养基上培养以检测MRSA。乳胶凝集试验证实可疑的MRSA分离物用于检测青霉素结合蛋白2a（PBP2a）。抗生素药敏试验通过Kirby Bauer纸片扩散法确定。在400个样本中有72个（18％）检测到金黄色葡萄球菌，其中52个（13％）被确认为MRSA。在原奶（200件中的37件； 18.5％）和鼻拭子（200件中的15件； 7.5％）中检出了耐甲氧西林金黄色葡萄球菌。没有显着性差异（p > 0.05），绵羊（37.7％）和山羊（23.4％）的MRSA患病率。MRSA分离株对氨苄西林（100％），氯沙西林（100％），磺胺甲恶唑-三甲氧苄啶（100％），阿莫西林-克拉维酸盐（84.6％），头孢曲松（75％），头孢呋辛（69.2％），红霉素（65.4％）有抗药性），链霉素（38.5％），环丙沙星（23.1％），培氟沙星（21.2％）和庆大霉素（17.3％）。在Abeokuta大都市饲养的小反刍动物中存在耐多药MRSA的原因可能是由于农民经常使用抗生素和不卫生的做法。反过来，这对小反刍动物产品的所有者，消费者和普通民众构成了潜在的公共健康风险。