Dark septate endophytes (DSE) are a group of multifunctional fungi that ubiquitously colonize the roots of numerous plants worldwide, benefiting the nutrition and adaptation of hosts to various stressed environments. How DSE benefit phosphorus absorption by their host plants remains unclear. We established pot cultures and compartmentalized microcosms in the greenhouse and used transcriptomic analysis to examine the response of maize to inoculation with a DSE strain, Exophiala pisciphila H93, which has been found to be capable of promoting plant growth and enhancing the tolerance of maize to heavy metals. H93 promoted the biomass, phosphorus absorption and photosynthesis of inoculated maize by enhancing phosphorus enzyme activities in the rhizosphere and by decreasing the pH of the rhizosphere compared with non-inoculated controls, either in sufficient or deficient phosphorus conditions in pot cultures. H93 inoculation changed the transcriptional pattern of maize related to 8 metabolic pathways. While H93 did not induce particular phosphate transporter gene expression under deficient phosphorus conditions, it dissolved the sparingly soluble phosphates (tricalcium phosphate) in the rhizosphere. Enhanced phosphorus absorption by the host plant by DSE (H93) seemed to occur independently of DSE-induced genes involved in phosphorus absorption. The greatest contribution of DSE to phosphorus utilization of hosts might be their efficiency in transferring sparingly soluble phosphorus into soluble phosphate (Pi).

中文翻译：

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暗分隔内生菌（Exophiala Pisciphila，H93）增强玉米幼苗对磷的吸收的证据

暗有隔内生菌 (DSE) 是一组多功能真菌，广泛存在于全球众多植物的根部，有利于宿主的营养和适应各种压力环境。DSE 如何促进寄主植物对磷的吸收尚不清楚。我们在温室中建立了盆栽培养和划分的微观世界，并使用转录组学分析来检查玉米对接种 DSE 菌株 Exophiala pisciphila H93 的反应，该菌株已被发现能够促进植物生长并增强玉米对重病的耐受性。金属。与未接种的对照相比，H93 通过增强根际磷酶活性和降低根际 pH 值来促进接种玉米的生物量、磷吸收和光合作用，在盆栽培养中磷充足或缺乏的条件下。H93 接种改变了与 8 条代谢途径相关的玉米的转录模式。虽然 H93 在缺磷条件下不会诱导特定的磷酸盐转运蛋白基因表达，但它会溶解根际中的微溶性磷酸盐（磷酸三钙）。DSE (H93) 增强宿主植物对磷的吸收似乎独立于参与磷吸收的 DSE 诱导基因。DSE 对宿主磷利用的最大贡献可能是它们将微溶性磷转化为可溶性磷酸盐 (Pi) 的效率。虽然 H93 在缺磷条件下不会诱导特定的磷酸盐转运蛋白基因表达，但它会溶解根际中的微溶性磷酸盐（磷酸三钙）。DSE (H93) 增强宿主植物对磷的吸收似乎独立于参与磷吸收的 DSE 诱导基因。DSE 对宿主磷利用的最大贡献可能是它们将微溶性磷转化为可溶性磷酸盐 (Pi) 的效率。虽然 H93 在缺磷条件下不会诱导特定的磷酸盐转运蛋白基因表达，但它会溶解根际中的微溶性磷酸盐（磷酸三钙）。DSE (H93) 增强宿主植物对磷的吸收似乎独立于参与磷吸收的 DSE 诱导基因。DSE 对宿主磷利用的最大贡献可能是它们将微溶性磷转化为可溶性磷酸盐 (Pi) 的效率。