Oxidative stress associated with chronic cerebral hypoperfusion is one of the fundamental factors leading to neurodegenerative diseases. To prevent oxidative stress, physical activity is effective. Physical exercise enables development of rehabilitation techniques that can progressively increase patients’ stress resistance. We determined the oxidative stress dynamics in experimental hypoperfusion and modeled rehabilitation measures, comparing sex and stress resistance levels. The experiment was performed on 240 Wistar rats of both sexes over a period of 90 days. Based on behavioral test results obtained using the open field test, the rats were divided into active animals with predicted higher stress resistance (HSR) and passive animals with predicted lower stress resistance (LSR). TBA (thiobarbituric acid) plasma concentration of the active products (malondialdehyde—MDA), blood plasma (NO-X) concentration, and l-citrulline (LC) concentration were determined spectrophotometrically at the corresponding wave length (nm). The intensity of oxidative stress was evaluated using the chemoluminscent method to determine the blood plasma antioxidant activity on the BCL-07 biochemoluminometer. This study revealed two stages of oxidative stress: a less pronounced phase covering the first days after surgery and a main one, which starts from the month after the operation to 3 months. Female sex and a high initial level of stress resistance reduced the severity of oxidative stress. Physical activity commencing a week after the surgery resulted in “reloading” the adaptive mechanisms and slowed the onset of the main stage, leading to a decrease in the free-radical process in all studied subgroups and the greater blood plasma (NO)-X decrease in the male animals. Future neuropharmacological intervention most likely will be able to determine the pathophysiology mechanism of chronic brain hypoperfusion and potentially extending adaptive responses.

Graphical abstract

与慢性脑灌注不足有关的氧化应激是导致神经退行性疾病的基本因素之一。为了防止氧化应激，体育锻炼是有效的。体育锻炼使康复技术得以发展，可以逐渐增加患者的压力抵抗力。我们确定了实验性灌注不足的氧化应激动态，并模拟了康复措施，比较了性别和应激抵抗水平。实验在90天的时间内对240只雌雄同体的Wistar大鼠进行。根据使用开放视野测试获得的行为测试结果，将大鼠分为具有较高预期抗应激能力（HSR）的活动动物和具有较低预期抗应激能力（LSR）的被动动物。升在相应的波长（nm）处通过分光光度法测定-瓜氨酸（LC）浓度。使用化学发光方法评估氧化应激的强度，以确定BCL-07生物化学光度计上的血浆抗氧化活性。这项研究揭示了氧化应激的两个阶段：一个不太明显的阶段，涵盖了手术后的第一天，另一个主要阶段，即从术后一个月到手术后3个月。女性和高水平的抗逆性降低了氧化应激的严重性。手术后一周开始的体育锻炼导致适应机制“重新加载”并减缓了主要阶段的发作，导致所有研究亚组的自由基过程减少，血浆（NO）-X降低更大在雄性动物中。

图形概要