Abstract

Objective: This study is designed to investigate the levels of carnitine and acylcarnitines (ACs) in the children with diabetes type 1 compared to the healthy subjects.

Methods: Forty-two type 1 diabetic children and healthy subjects were recruited in the study, respectively. In addition to FBS and Hb A1C, free carnitine and ACs in butyl-ester form in the fasting blood samples were assessed by isotope dilution mass spectrometry for all diabetics and controls using the tandem mass spectrometry system.

Results: Diabetic patients had a higher level of C, C4, C6, C14, C18:2, and C18:2OH. Females had elevated C14:2 compared to the males. The C18:2 and C18:2OH levels were elevated as the Hb A1C level increased. The C18:2, C14OH were mostly increased in the prediabetic and diabetic patients, respectively.

Conclusion: Increased ACs level indicates the increased acyl-CoA intermediates for the fatty acids and amino acids oxidation.

摘要

目的：本研究旨在调查与健康受试者相比，1 型糖尿病儿童的肉碱和酰基肉碱 (ACs) 水平。

方法：本研究分别招募了42名1型糖尿病儿童和健康受试者。除了 FBS 和 Hb A1C 外，使用串联质谱系统通过同位素稀释质谱法对所有糖尿病患者和对照组的空腹血样中的游离肉碱和丁酯形式的 ACs 进行了评估。

结果：糖尿病患者的 C、C4、C6、C14、C18:2 和 C18:2OH 水平较高。与男性相比，女性的 C14:2 升高。随着 Hb A1C 水平升高，C18:2 和 C18:2OH 水平升高。C18:2、C14OH 分别在糖尿病前期和糖尿病患者中增加最多。

结论：增加的 ACs 水平表明增加了用于脂肪酸和氨基酸氧化的酰基辅酶 A 中间体。