A novel method for the analysis of nalbuphine hydrochloride (NAL) is reported based on its enhancement effect on a diperiodatoargentate(III)-rhodamine-B (Ag(III) complex-Rh-B) chemiluminescence (CL) system in an aqueous sulfuric acid medium using flow-injection analysis (FIA). The optimal conditions of the CL reaction were: sulfuric acid, 10⁻² M; Ag(III) complex, 2.0 × 10⁻⁴ M; Rh-B, 2.0 × 10⁻⁵ M; Brij-35, 0.01%; sample loop volume, 300 μL; and flow rate, 3.0 mL/min/stream. The limit of detection (LOD) and limit of quantification (LOQ) were 0.001 and 0.003 mg/L (S/N = 3 and 10); linear calibration range, 5 × 10⁻³ – 5.0 mg/L (R² = 0.9999) and injection throughput, 150/h. The relative standard deviation (RSD) was from 0.8 – 3.2% over the range studied. The suggested technique was applied for the determination of NAL in pharmaceutical injections, compared with a reported spectrophotometric method, and obtained results were found to be satisfactory. Based on spectrophotometric studies, the most probable mechanism of the CL reaction has been briefly described and drawn schematically.

报道了一种分析盐酸纳布啡 (NAL) 的新方法，该方法基于其对硫酸水溶液中二过碘银酸盐 (III)-罗丹明 -B (Ag(III) 复合物 -Rh-B) 化学发光 (CL) 体系的增强作用使用流动注射分析（FIA）的介质。CL反应的最佳条件为：硫酸，10 ^-2 M；Ag(III)络合物，2.0 × 10 ^-4 M；Rh-B，2.0×10 ^-5 M；Brij-35，0.01%；样品环体积，300 μL；和流速，3.0 mL/min/stream。检测限（LOD）和定量限（LOQ）分别为0.001和0.003 mg/L（S/N = 3和10）；线性校准范围，5 × 10 ^-3 – 5.0 mg/L ( R ² = 0.9999)，进样量，150/h。在研究范围内，相对标准偏差 (RSD) 为 0.8 – 3.2%。将所提出的技术应用于药物注射剂中NAL的测定，与已报道的分光光度法进行比较，结果令人满意。基于分光光度研究，我们对 CL 反应最可能的机理进行了简要描述并绘制了示意图。