In present study the effect of iron (Fe) and manganese (Mn) contamination was assessed by modeling a freshwater food web of water, zooplankton (Daphnia pulex), and zebrafish (Danio rerio) under laboratory conditions. Metals were added to the rearing media of D. pulex, and enriched zooplankton was fed to zebrafish in a feeding trial. The elemental analysis of rearing water, zooplankton, and fish revealed significant difference in the treatments compared to the control. In D. pulex the Mn level increased almost in parallel with the dose of supplementation, as well as the Fe level differed statistically. A negative influence of the supplementation on the fish growth was observed: specific growth rate (SGR%) and weight gain (WG) decreased in Fe and Mn containing treatments. The redundancy analysis (RDA) of concentration data showed strong correlation between the rearing water and D. pulex, as well as the prey organism of Fe- and Mn-enriched D. pulex and the predator organism of D. rerio. The bioconcentration factors (BCF) calculated for water to zooplankton further proved the relationship between the Fe and Mn dosage applied in the treatments and measured in D. pulex. Trophic transfer factor (TTF) results also indicate that significant retention of the metals occurred in D. rerio individuals, however, in a much lower extent than in the water to zooplankton stage. Our study suggests that Fe and Mn significantly accumulate in the lower part of the trophic chain and retention is effective through the digestive track of zebrafish, yet no biomagnification occurs.

在本研究中，通过在实验室条件下对淡水食物网，浮游动物（Daphnia pulex）和斑马鱼（Danio rerio）的淡水食物网进行建模，评估了铁（Fe）和锰（Mn）污染的影响。金属加入到饲养介质D.状溞，和富集的浮游动物送入斑马鱼在饲养试验。饲养水，浮游动物和鱼类的元素分析显示，与对照组相比，处理方法存在显着差异。在D. pulex锰含量几乎与补充剂量平行增加，而铁含量也有统计学差异。观察到补充剂对鱼的生长有不利影响：含铁和锰的处理的比生长率（SGR％）和增重（WG）降低。浓度数据的冗余分析（RDA）显示，饲养水和D. pulex以及富含Fe和Mn的D. pulex的猎物生物与D. rerio的捕食生物之间具有很强的相关性。计算出的水与浮游动物的生物富集因子（BCF）进一步证明了在处理中使用的铁和锰剂量之间的关系以及在D. pulex中进行了测量。营养传递因子（TTF）结果还表明，金属的显着保留发生在D. rerio个体中，但是其程度远低于从水到浮游动物的阶段。我们的研究表明，Fe和Mn在营养链的下部大量积聚，并且在斑马鱼的消化道中保持有效，但未发生生物放大作用。