Centrosomes are highly conserved organelles that act as the major microtubule-organizing center (MTOC) in animal somatic cells. Through their MTOC activity, centrosomes play various roles throughout the cell cycle, such as supporting cell migration in interphase and spindle organization and positioning in mitosis. Various approaches for removing centrosomes from somatic cells have been developed and applied over the past few decades to understand the precise roles of centrosomes. Centrinone, a reversible and selective PLK4 (polo-like kinase 4) inhibitor, has recently emerged as an efficient approach to eliminate centrosomes. In this review, we describe the latest findings on centrosome function that have been revealed using various centrosome-eliminating approaches. In addition, we discuss our recent findings on the mechanism of centrosome-independent spindle bipolarization, discovered through the use of centrinone.Key words: centrosome, centrinone, mitotic spindle, bipolarity, NuMA.

中文翻译：

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通过消除中心体出现中心体和非中心体功能。


中心体是高度保守的细胞器，充当动物体细胞中的主要微管组织中心（MTOC）。通过其 MTOC 活性，中心体在整个细胞周期中发挥各种作用，例如支持间期和纺锤体组织中的细胞迁移以及有丝分裂中的定位。在过去的几十年里，已经开发并应用了各种从体细胞中去除中心体的方法，以了解中心体的精确作用。 Centrinone 是一种可逆的选择性 PLK4（polo 样激酶 4）抑制剂，最近已成为消除中心体的有效方法。在这篇综述中，我们描述了使用各种中心体消除方法揭示的中心体功能的最新发现。此外，我们还讨论了我们最近通过使用centrinone发现的不依赖于中心体的纺锤体双极化机制的发现。关键词：中心体，centrinone，有丝分裂纺锤体，双极性，NuMA。