Early-life lactoferrin intervention modulates the colonic microbiota, colonic microbial metabolites and intestinal function in suckling piglets.,Applied Microbiology and Biotechnology

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Early-life lactoferrin intervention modulates the colonic microbiota, colonic microbial metabolites and intestinal function in suckling piglets.
Applied Microbiology and Biotechnology ( IF 3.9 ) Pub Date : 2020-05-22 , DOI: 10.1007/s00253-020-10675-z
Ping Hu ₁ , Fangzhou Zhao ₁ , Jing Wang ₁ , Weiyun Zhu ₁

Affiliation

This study reports the effects of early-life lactoferrin (LF) intervention on the colonic microbiota, intestinal function and mucosal immunity in suckling piglets. A total of 60 Duroc × Landrace × Yorkshire suckling piglets from six sows were assigned to the control (CON) and LF groups in litters. The LF group piglets were fed 0.5 g/kg body weight of LF solution per day, and the CON group piglets were fed the same dose of physiological saline for a week. Six piglets from the two groups were randomly chosen and euthanised on days 8 and 21. The LF group piglets had higher ACE and Chao1 indices of colonic microbiota than the CON group piglets (P < 0.05). In addition, the LF group piglets had a higher abundance of Roseburia (P < 0.05) and a lower abundance of Escherichia–Shigella (P < 0.05) in the colonic digesta. The LF group piglets also had a higher concentration of butyrate (P < 0.05) in the colonic digesta. Moreover, the LF group piglets had a higher gene expression of occludin (P < 0.05) in the colonic mucosa. In addition, the gene expression of MUC4 was upregulated in the LF group piglets compared with that in the CON group on day 21 (P < 0.05), and the lower gene expression of TLR-4 was found in the LF group compared with the CON group on day 8 (P < 0.05). Furthermore, the concentration of IL-10 was increased in the LF group on day 8 (P < 0.05), while the LF group piglets had a higher concentration of sIgA and lower concentrations of IL-1α and IL-1β (P < 0.05) in the colonic mucosa. These results suggest that early-life LF intervention can modulate the composition of colonic microbiota and improve the intestinal function in suckling piglets.

Key Points
• Early-life LF intervention significantly modulated colon microbiota.
• Early-life LF intervention can improve the colon health.
• The colon microbiota plays an important role in host health.

中文翻译：

早期乳铁蛋白干预可调节乳猪的结肠微生物群，结肠微生物代谢产物和肠道功能。

这项研究报告了早期乳铁蛋白（LF）干预对乳猪结肠微生物群，肠道功能和粘膜免疫的影响。将来自六头母猪的60头杜洛克×长白×约克夏乳猪分配到对照组（CON）和LF组。每天给LF组仔猪饲喂0.5 g / kg体重的LF溶液，向CON组仔猪饲喂相同剂量的生理盐水一周。从第8天和第21天随机选择两组中的6只仔猪并实施安乐死。LF组仔猪的结肠菌群的ACE和Chao1指数高于CON组仔猪（P <0.05）。此外，LF组仔猪的玫瑰菌丰度较高（P <0.05），而玫瑰猪丰度较低。结肠消化物中的大肠杆菌（Escherichia–Shigella）（P <0.05）。LF组仔猪结肠消化物中丁酸盐的浓度也较高（P <0.05）。此外，LF组仔猪在结肠黏膜中的occludin基因表达较高（P <0.05）。此外，与第21天的CON组相比，LF组仔猪的MUC4基因表达上调（P <0.05），而LF组的TLR-4基因表达较CON组低。组在第8天（P <0.05）。此外，在第8天，LF组的IL-10浓度升高（P <0.05），而LF组仔猪结肠黏膜中的sIgA浓度较高，而IL-1α和IL-1β的浓度较低（P <0.05）。这些结果表明，早期的LF干预可以调节乳猪的结肠微生物群的组成并改善肠道功能。