Applied Microbiology and Biotechnology ( IF 3.9 ) Pub Date : 2020-05-22 , DOI: 10.1007/s00253-020-10675-z Ping Hu 1 , Fangzhou Zhao 1 , Jing Wang 1 , Weiyun Zhu 1
This study reports the effects of early-life lactoferrin (LF) intervention on the colonic microbiota, intestinal function and mucosal immunity in suckling piglets. A total of 60 Duroc × Landrace × Yorkshire suckling piglets from six sows were assigned to the control (CON) and LF groups in litters. The LF group piglets were fed 0.5 g/kg body weight of LF solution per day, and the CON group piglets were fed the same dose of physiological saline for a week. Six piglets from the two groups were randomly chosen and euthanised on days 8 and 21. The LF group piglets had higher ACE and Chao1 indices of colonic microbiota than the CON group piglets (P < 0.05). In addition, the LF group piglets had a higher abundance of Roseburia (P < 0.05) and a lower abundance of Escherichia–Shigella (P < 0.05) in the colonic digesta. The LF group piglets also had a higher concentration of butyrate (P < 0.05) in the colonic digesta. Moreover, the LF group piglets had a higher gene expression of occludin (P < 0.05) in the colonic mucosa. In addition, the gene expression of MUC4 was upregulated in the LF group piglets compared with that in the CON group on day 21 (P < 0.05), and the lower gene expression of TLR-4 was found in the LF group compared with the CON group on day 8 (P < 0.05). Furthermore, the concentration of IL-10 was increased in the LF group on day 8 (P < 0.05), while the LF group piglets had a higher concentration of sIgA and lower concentrations of IL-1α and IL-1β (P < 0.05) in the colonic mucosa. These results suggest that early-life LF intervention can modulate the composition of colonic microbiota and improve the intestinal function in suckling piglets.
Key Points • Early-life LF intervention significantly modulated colon microbiota. • Early-life LF intervention can improve the colon health. • The colon microbiota plays an important role in host health. |
中文翻译:
早期乳铁蛋白干预可调节乳猪的结肠微生物群,结肠微生物代谢产物和肠道功能。
这项研究报告了早期乳铁蛋白(LF)干预对乳猪结肠微生物群,肠道功能和粘膜免疫的影响。将来自六头母猪的60头杜洛克×长白×约克夏乳猪分配到对照组(CON)和LF组。每天给LF组仔猪饲喂0.5 g / kg体重的LF溶液,向CON组仔猪饲喂相同剂量的生理盐水一周。从第8天和第21天随机选择两组中的6只仔猪并实施安乐死。LF组仔猪的结肠菌群的ACE和Chao1指数高于CON组仔猪(P <0.05)。此外,LF组仔猪的玫瑰菌丰度较高(P <0.05),而玫瑰猪丰度较低。 结肠消化物中的大肠杆菌(Escherichia–Shigella)(P <0.05)。LF组仔猪 结肠消化物中丁酸盐的浓度也较高(P <0.05)。此外,LF组仔猪 在结肠黏膜中的occludin基因表达较高(P <0.05)。此外,与第21天的CON组相比,LF组仔猪的MUC4基因表达上调(P <0.05),而LF组的TLR-4基因表达较CON组低。组在第8天(P <0.05)。此外,在第8天,LF组的IL-10浓度升高(P <0.05),而LF组仔猪 结肠黏膜中的sIgA浓度较高,而IL-1α和IL-1β的浓度较低(P <0.05)。这些结果表明,早期的LF干预可以调节乳猪的结肠微生物群的组成并改善肠道功能。
要点 •早期生命LF干预显着调节了结肠菌群。 •早期LF干预可以改善结肠健康。 •结肠微生物群在宿主健康中起重要作用。 |