Industrial effluents are potential threats to water bodies owing to the chemicals they contain to create pollution. Activated sludge effluents represent an ecosystem of multifaceted and interactive microbial consortia that flourish with different genetic as well as metabolic potential. This study is applied to a nanopore sequencing to understand the microbial organisms involved in degradation pathways, and their interaction among them. The taxonomic profile determined the occurrence of Proteobacteria, Actinobacteria, Terrabacteria, bacteria, and Firmicutes with the dominance of Burkholderiales. Burkholderia pseudomallei was found to be 13.79% of the classified reads. The Gene ontology evaluation revealed that mobile elements and tRNA genes were the predominant genetic composition of the community with 16% of the total genes. The functional annotation of genes ensured the presence of genes encoding various types of RNA, homo and heteromultimeric proteins and pseudogenes as well. Pathways that involved in effluent treatment such as amines, aromatic, organic, and aromatic compound degradation were also illustrated. Amino acyl tRNA charging, signal transduction, and hormone degradation were the pathways that are identified to be common in all the organisms of the activated sludge. The study elaborated on the xenobiotic degradation that is exclusive of the predominant Burkholderiales and the interaction of them in the community as a whole. The present study revealed the genes, proteins, and the pathways of the activated sludge microbiome which will enhance the perceptions of the core mechanisms of effluent degradation.

中文翻译：

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通过宏基因组分析去除厌氧反应器纺织废水处理中活性污泥的微生物相互作用

由于工业废水中含有会造成污染的化学物质，因此它们对水体构成潜在威胁。活性污泥流出物代表了一个由多方面和互动的微生物群落组成的生态系统，它们以不同的遗传和代谢潜力蓬勃发展。这项研究应用于纳米孔测序，以了解参与降解途径的微生物及其之间的相互作用。分类学特征确定了变形菌门、放线菌门、土菌门、细菌和厚壁菌门的出现，其中伯克霍尔德菌门占优势。发现 Burkholderia pseudomallei 占分类读数的 13.79%。基因本体评估显示，移动元件和 tRNA 基因是群落的主要遗传组成，占总基因的 16%。基因的功能注释确保存在编码各种类型的 RNA、同源和异源多聚体蛋白质以及假基因的基因。还说明了参与废水处理的途径，例如胺、芳香族、有机和芳香族化合物的降解。氨基酰基 tRNA 充电、信号转导和激素降解是活性污泥中所有生物体中常见的途径。该研究详细阐述了不包括主要伯克霍尔德氏菌的异生物质降解以及它们在整个社区中的相互作用。本研究揭示了活性污泥微生物组的基因、蛋白质和途径，这将增强对污水降解核心机制的认识。