Purpose

In this study, we aimed to determine the regenerative and antimicrobial impact of the electrospun nanofiber mats, with/without silver nanoparticles (AgNPs), on full-thickness skin wounds in rabbits.

Methods

Polyvinyl alcohol was combined with gelatin to provide biocompatible electrospun binary nanofiber mats. AgNPs were added to the polyvinyl alcohol/gelatin mixture to obtain ternary nanofiber-AgNPs mats. Binary and ternary nanofiber mats were characterized by scanning electron microscopy before being applied as wound dressings in vivo. Subsequently, wound healing was evaluated.

Results

Both nanofiber/nanofiber-AgNPs mats improved the microscopic quality of the healed skin, albeit without obvious acceleration of the healing rate. As well, both types of nanofiber mats were able to combat microbial invasion into the wound bed.

Conclusions

Both binary polyvinyl alcohol/gelatin and ternary polyvinyl alcohol/gelatin/AgNPs nanofiber mats developed in the present study depicted similar regenerative and antimicrobial potential when applied as full-thickness wound dressing. However, in comparison to the binary nanofiber mats, no obvious synergistic effect was observed after loading nanofibers with AgNPs.

中文翻译：

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用于伤口愈合应用的银纳米颗粒/聚乙烯醇/明胶三元纳米纤维垫的制备。

目的

在这项研究中，我们旨在确定电纺纳米纤维垫（含/不含银纳米粒子（AgNP））对兔全层皮肤伤口的再生和抗菌影响。

方法

聚乙烯醇与明胶结合以提供生物相容性电纺二元纳米纤维垫。将 AgNPs 添加到聚乙烯醇/明胶混合物中以获得三元纳米纤维-AgNPs 垫。二元和三元纳米纤维垫在用作体内伤口敷料之前通过扫描电子显微镜进行表征。随后，评估伤口愈合。

结果

纳米纤维/纳米纤维-AgNPs 垫都改善了愈合皮肤的微观质量，尽管没有明显加快愈合速度。同样，两种类型的纳米纤维垫都能够抵抗微生物侵入伤口床。

结论

本研究中开发的二元聚乙烯醇/明胶和三元聚乙烯醇/明胶/AgNPs 纳米纤维垫在用作全层伤口敷料时表现出相似的再生和抗菌潜力。然而，与二元纳米纤维垫相比，在纳米纤维上加载 AgNPs 后没有观察到明显的协同效应。