The Palette of Techniques for Cell Cycle Analysis,FEBS Letters

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The Palette of Techniques for Cell Cycle Analysis
FEBS Letters ( IF 3.0 ) Pub Date : 2020-06-03 , DOI: 10.1002/1873-3468.13842
Anna E Eastman ₁ , Shangqin Guo ₁

Affiliation

The cell division cycle is the generational period of cellular growth and propagation. Cell cycle progression needs to be highly regulated to preserve genomic fidelity while increasing cell number. In multicellular organisms, the cell cycle must also coordinate with cell fate specification during development and tissue homeostasis. Altered cell cycle dynamics play a central role also in a number of pathophysiological processes. Thus, extensive effort has been made to define the biochemical machineries that execute the cell cycle and their regulation, as well as implementing more sensitive and accurate cell cycle measurements. Here, we review the available techniques for cell cycle analysis, revisiting the assumptions behind conventional population‐based measurements and discussing new tools to better address cell cycle heterogeneity in the single‐cell era. We weigh the strengths, weaknesses, and trade‐offs of methods designed to measure temporal aspects of the cell cycle. Finally, we discuss emerging techniques for capturing cell cycle speed at single‐cell resolution in live animals.

中文翻译：

细胞周期分析技术组合

细胞分裂周期是细胞生长和繁殖的世代时期。细胞周期进程需要受到高度调控，以在增加细胞数量的同时保持基因组保真度。在多细胞生物中，细胞周期还必须与发育和组织稳态过程中的细胞命运规范相协调。细胞周期动力学的改变在许多病理生理过程中也发挥着核心作用。因此，人们做出了大量的努力来定义执行细胞周期及其调节的生化机制，以及实施更灵敏和更准确的细胞周期测量。在这里，我们回顾了细胞周期分析的可用技术，重新审视了传统的基于群体的测量背后的假设，并讨论了更好地解决单细胞时代细胞周期异质性的新工具。我们权衡了旨在测量细胞周期时间方面的方法的优点、缺点和权衡。最后，我们讨论了以单细胞分辨率捕获活体动物细胞周期速度的新兴技术。

更新日期：2020-06-03

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