Deoxynivalenol (DON) is commonly found in wheat and wheat-derived foods, posing a threat to human health. Biodegradation is an efficient and eco-friendly measure for mycotoxin detoxification. Understanding the mechanism of DON biodegradation is hence of great importance. Herein, we report the application of metabolomics methods for the analysis of DON degradation by a bacterial consortium isolated from wheat leaves collected in Jiangsu Province. Metabolomics analysis combined with a nuclear magnetic resonance analysis revealed the main degradation product, 3-keto-DON, and a minor degradation product, 3-epi-DON. Further study illustrated that DON underwent a two-step epimerization through the intermediate 3-keto-DON. Sequencing analysis of the 16S rRNA metagenome of the microorganismal community suggested that the abundance of three bacterial genera, Achromobacter, Sphingopyxis, and Sphingomonas, substantially increased during the coculture of bacterial consortium and DON. Further investigation revealed that Devosia sp. might be responsible for the epimerization of 3-keto-DON. These findings shed light on the catabolic pathways of DON during biodegradation and illustrate the potential of using metabolomics approaches in biodegradation studies.

Key Points

• A bacterial consortium was isolated with good deoxynivalenol-degrading potential.

• Metabolomics approaches were successfully used to interpret the degradation pathway.

• A trace-amount degradation product was determined by metabolomics and NMR analysis.

.

脱氧雪腐烯酚（DON）通常存在于小麦和小麦衍生食品中，对人体健康构成威胁。生物降解是一种高效，环保的霉菌毒素排毒措施。因此，了解DON生物降解的机制非常重要。本文中，我们报道了代谢组学方法在分析江苏省小麦叶片中分离的细菌组成的DON降解中的应用。代谢组学分析与核磁共振分析相结合，揭示了主要降解产物3-keto-DON和次要降解产物3-epi-DON。进一步的研究表明，DON通过中间的3-酮-DON经历了两步差向异构化。无色杆菌，鞘脂单胞，和鞘氨醇，细菌聚生和DON的共培养物中显着增加。进一步的调查显示，Devosia sp。可能是3-酮-DON的差向异构化的原因。这些发现揭示了DON在生物降解过程中的分解代谢途径，并说明了在生物降解研究中使用代谢组学方法的潜力。

关键点

•分离出具有良好脱氧雪腐烯醇降解潜能的细菌聚生体。

•代谢组学方法已成功用于解释降解途径。

•通过代谢组学和NMR分析确定了痕量降解产物。

。