Tissue regeneration often requires the use of biocompatible resorbable scaffolds to support the ingrowth of cells from neighboring tissues into a localized tissue defect. Such scaffolds must possess surface molecular cues that stimulate cells to populate the device, the first necessary condition for the formation of a healthy tissue. Chitosan is a natural polymer that has long been tested in biomedical applications because of its high biocompatibility, which can be further increased by modifying its formulation, e.g. adding D-(+) raffinose. We used this formulation in an ad hoc designed 3D printer to create regularly ordered scaffolds, which we then enriched with type IV collagen, an isoform of collagen that is exclusively found in basement membranes. Human epithelial A549 cells were then seeded on control scaffolds or on scaffolds coated with collagen, which was precipitated, or on scaffolds first collagenized and then exposed to either UVB or UVC radiation. Observations by the...

中文翻译：

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用 D-(+) 棉子糖修饰并富含 IV 型胶原蛋白以改善上皮细胞定植的 3D 打印壳聚糖支架。

组织再生通常需要使用生物相容性可吸收支架来支持细胞从邻近组织向内生长到局部组织缺损。这种支架必须具有表面分子线索，刺激细胞填充设备，这是形成健康组织的第一个必要条件。壳聚糖是一种天然聚合物，长期以来一直在生物医学应用中进行测试，因为它具有高生物相容性，可以通过修改其配方进一步提高生物相容性，例如添加 D-(+) 棉子糖。我们在特别设计的 3D 打印机中使用这种配方来创建规则有序的支架，然后我们用 IV 型胶原蛋白对其进行富集，这是一种仅存在于基底膜中的胶原蛋白同种型。然后将人上皮 A549 细胞接种在对照支架或涂有胶原蛋白的支架上，胶原蛋白沉淀，或在首先胶原化然后暴露于 UVB 或 UVC 辐射的支架上。来自...的观察