The precise positioning and arrangement of cell spheroids and organoids are critical to reconstructing complex tissue architecture for tissue engineering and regenerative medicine. Here, we present a digital acoustofluidic method to manipulate cell spheroids and organoids with unprecedented dexterity. By introducing localized vibrations via a C-shaped integrated digital transducer (IDT), we can generate a trapping node to immobilize cell spheroids with a diameters ranging from 20 μ m to 300 μ m. Moreover, we digitally trapped multiple cell spheroids atop the C-shaped IDTs within a closed or open microfluidic chamber. By programming the trapping nodes within a 3 × 3 C-shaped IDT array, we can precisely position cell spheroids into designed patterns. We also demonstrated that our digital acoustofluidic device can accurately control the interaction of spheroid cells and organoids. Along with a simple fabrication procedure and setup, our digital acoustofluidic method can p...

中文翻译：

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使用数字声流技术捕获细胞球体和类器官。

细胞球体和类器官的精确定位和布置对于重建用于组织工程和再生医学的复杂组织结构至关重要。在这里，我们提出了一种数字声流方法，以前所未有的灵活性来操纵细胞球体和类器官。通过C型集成数字换能器（IDT）引入局部振动，我们可以生成一个捕获节点来固定直径为20μm至300μm的细胞球体。此外，我们在封闭或开放的微流体室内，将C型IDT顶部的多个细胞球体数字化捕获。通过对3×3 C形IDT阵列中的捕获节点进行编程，我们可以将细胞球体精确定位到设计的图案中。我们还证明了我们的数字声流设备可以准确地控制球状细胞和类器官的相互作用。除了简单的制造程序和设置，我们的数字声流方法还可以...