This paper reports the use of near infrared and mid-infrared spectroscopy to detect the presence and quantity of eggs of the gastrointestinal nematode Haemonchus contortus in sheep faeces. Haemonchus contortus eggs were quantified in dried, finely ground sheep faeces and in moist, coarsely ground faeces using near infrared and mid-infrared bench top spectrometers and a portable near infrared spectrometer. When Haemonchus contortus eggs were presented without faecal medium, it was found that the wavelength region of 1880–2100 nm was most important for detection. Broad classes of chemical properties found in the near infrared region were identified for dried Haemonchus contortus eggs using a mid-infrared spectrometer. However, when Haemonchus contortus eggs were mixed into the complex matrix of sheep faeces, the development of a robust calibration model for egg detection proved to be challenging (R2 < 0.47). The reliability of this method, if used for the detection of Haemonchus contortus eggs in the field, may be further limited by variations in egg species, faecal moisture content, faecal composition and particle size. Nevertheless, this is the first report identifying near infrared bands for Haemonchus contortus eggs and provides valuable information for future studies towards a spectroscopy-based method for detection of gastrointestinal nematodes.

中文翻译：

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使用近红外和中红外光谱法检测绵羊粪便中的捻线虫卵

本文报道了使用近红外和中红外光谱检测绵羊粪便中胃肠线虫扭曲血牙线虫卵的存在和数量。使用近红外和中红外台式光谱仪和便携式近红外光谱仪对干燥的、细磨的绵羊粪便和潮湿的、粗磨的粪便中的 Haemonchus 卵进行定量。当在没有粪便培养基的情况下提供旋涡血球虫卵时，发现 1880-2100 nm 的波长区域对检测最重要。使用中红外光谱仪鉴定了干旋涡旋血球虫卵在近红外区域发现的多种化学特性。然而，当捻线虫卵混入绵羊粪便的复杂基质中时，开发用于鸡蛋检测的稳健校准模型被证明具有挑战性（R2 < 0.47）。这种方法的可靠性，如果用于现场检测扭曲血霉的卵，可能会进一步受到卵种类、粪便水分含量、粪便成分和颗粒大小变化的限制。尽管如此，这是第一份确定扭曲血霉卵的近红外波段的报告，并为未来研究基于光谱的胃肠道线虫检测方法提供了有价值的信息。