Cowpea is one of the most important legume grain in the SubSaharian region of Africa used for human consumption and animal feeding but its production is hampered by biotic and abiotic constraints raising the need to broaden its genetic basis. For this purpose, the seeds of two cowpea varieties Melakh and Yacine were respectively irradiated with 300 and 340 Gy. The developed mutant populations were agromorphologically characterized from M5 to M7 while the genetic diversity of the last were evaluated using 13 ISSR markers. Based on agromorphological characterization, variation of flower color, pod length, seed coat color and seed weight with respectively 78.01, 68.29, 94.48 and 57.58% heritability were recorded in the mutant lines. PCA analyses allowed to identify the elite mutants based on their agromorphological traits while Pearson s correlation results revealed a positive correlation between yield component traits. Three subpopulations were identified through STRUCTURE analyses but assignment of the individuals in each group was improved using DAPC. Analysis of Molecular Variance revealed that the majority (85%) of the variance rather existed within group than among (15%) group. Finally, our study allowed to select new promising mutant genotypes which could be tested for multi local trials to evaluate their agronomic performance.

中文翻译：

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新cow豆（Vigna unguiculata）突变基因型的开发，农艺变异，遗传多样性和种群结构分析

pea豆是非洲撒哈拉以南地区最重要的豆类谷物之一，用于人类食用和动物饲养，但由于生物和非生物限制，its豆的生产受到限制，因此有必要扩大其遗传基础。为此，分别用300和340 Gy辐射两个cow豆品种Melakh和Yacine的种子。从M5到M7对已发育的突变种群进行了农业形态学表征，同时使用13个ISSR标记评估了最后一个种群的遗传多样性。根据农艺学特征，突变株系记录花色，豆荚长度，种皮颜色和种子重量的变异性分别为78.01、68.29、94.48和57.58％。PCA分析可以根据其突变体的农艺性状鉴定出优良突变体，而Pearson的相关性结果则表明产量成分性状之间具有正相关性。通过结构分析确定了三个亚群，但使用DAPC改善了每个组中个体的分配。分子方差分析表明，大多数差异（85％）在组内存在，而不是组内（15％）。最后，我们的研究允许选择新的有前途的突变体基因型，可以对其进行多本地试验以评估其农艺性能。分子方差分析表明，大多数差异（85％）在组内存在，而不是组内（15％）。最后，我们的研究允许选择新的有前途的突变体基因型，可以对其进行多本地试验以评估其农艺性能。分子方差分析表明，大多数差异（85％）在组内存在，而不是组内（15％）。最后，我们的研究允许选择新的有前途的突变体基因型，可以对其进行多本地试验以评估其农艺性能。