Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.

中文翻译：

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jYCaMP：一种优化的钙指示剂，用于光纤激光波长下的双光子成像。

固定波长高于 1,000 nm 的飞秒激光器功能强大、稳定且廉价，使其成为双光子显微镜的有前途的光源。下一代显微镜和经济实惠的交钥匙系统都需要针对这些波长进行优化的生物传感器。在这里，我们报告了 jYCaMP1，它是钙指示剂 jGCaMP7 的黄色变体，在小鼠中表现优于其亲代，并且在激发波长高于 1,000 nm 时飞行，并且能够使用基于红色荧光蛋白的指示剂改进双色钙成像。