当前位置: X-MOL 学术Int. J. Neurosci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Dynamic alteration of dendrites and dendritic spines in the hippocampus and microglia in mouse brain tissues after kainate-induced status epilepticus
International Journal of Neuroscience ( IF 1.7 ) Pub Date : 2020-06-01 , DOI: 10.1080/00207454.2020.1770246
Lingling Xie 1, 2, 3, 4, 5, 6 , Tianyi Li 1, 3, 4, 5, 6 , Xiaojie Song 1, 3, 4, 5, 6 , Hong Sun 1, 3, 4, 5, 6 , Jie Liu 1, 3, 4, 5, 6 , Jing Yang 1, 3, 4, 5, 6 , Wenjie Zhao 1, 3, 4, 5, 6 , Li Cheng 2, 3, 4, 5, 6 , Hengsheng Chen 2, 3, 4, 5, 6 , Benke Liu 1, 3, 4, 5, 6 , Wei Han 1, 3, 4, 5, 6 , Chen Yang 1, 3, 4, 5, 6 , Li Jiang 1, 2, 3, 4, 5, 6
Affiliation  

Abstract

Purpose

To study the alteration of microglial subtypes, the representative markers of microglia, and the morphology of dendrites and dendritic spines after acute status epilepticus (SE) and during recurrent seizures.

Methods

A mouse kainate-induced SE model was used. Dendrites and dendritic spines of granule neurons in the dentate gyrus (DG) subregion and pyramidal neurons in the cornu ammonis (CA)1 and cornu ammonis (CA)3 subregions of the hippocampus were visualized by Golgi staining. Synaptic proteins were evaluated by Western blot analysis, and microglia and their markers were evaluated by flow cytometry.

Results

Extensive partial spine loss was observed in the dendrites of granule and pyramidal cells in the acute and early chronic stages of SE. In terms of spine loss, the thin and mushroom types predominated. Accompanying the spine loss in these two stages, the proportion of M1 microglia increased significantly with high CX3CR1 expression and low CD200R expression. However, at the transiting stage, the proportion of M2 microglia was increased dramatically, and high expression levels of CXCR3 on all microglia and CD68 on M1 microglia were observed. Morris water maze tests revealed significant learning and memory impairment in the chronic phase of epilepsy.

Conclusion

Dendritic spines in the hippocampus and microglia in the central nevus system are dynamically altered in epilepsy during the establishment and maintenance of spontaneous seizures. Microglia may contribute to the spine loss and related learning and memory impairment.



中文翻译:

红藻氨酸诱发癫痫持续状态后小鼠脑组织海马和小胶质细胞树突和树突棘的动态改变

摘要

目的

研究急性癫痫持续状态(SE)和反复发作期间小胶质细胞亚型的改变、小胶质细胞的代表性标志物以及树突和树突棘的形态。

方法

使用小鼠红藻氨酸诱导的 SE 模型。通过高尔基体染色观察海马齿状回 (DG) 亚区颗粒神经元的树突和树突棘,以及海马角 (CA)1 和角叶 (CA)3 亚区的锥体神经元。通过蛋白质印迹分析评估突触蛋白,并通过流式细胞术评估小胶质细胞及其标志物。

结果

在 SE 的急性和早期慢性阶段,在颗粒和锥体细胞的树突中观察到广泛的部分脊柱丧失。在脊椎损失方面,薄型和蘑菇型占主导地位。伴随这两个阶段的脊柱缺失,M1小胶质细胞的比例显着增加,CX3CR1高表达和CD200R低表达。然而,在过渡阶段,M2小胶质细胞的比例显着增加,并且观察到所有小胶质细胞上CXCR3和M1小胶质细胞上CD68的高表达水平。莫里斯水迷宫测试显示癫痫慢性期有显着的学习和记忆障碍。

结论

海马中的树突棘和中央痣系统中的小胶质细胞在癫痫的建立和维持期间会发生动态变化。小胶质细胞可能导致脊柱丧失和相关的学习和记忆障碍。

更新日期:2020-06-01
down
wechat
bug