Abstract

The ethyl acetate fraction of the dried aerial parts of Senecio glaucus L. exhibited significant antimicrobial activity against some of selected bacteria and fungi. Also, it showed potent cytotoxicity against PANC-1 cancer cell lines under glucose deficient medium. The ethyl acetate fraction was subjected to different chromatographic techniques for isolation of the bioactive compounds. A new benzofuran glucoside; 2,3-dihydro-3β-hydroxyeuparin 3-O-glucopyranoside (1) was isolated. Additionally, two known flavonoid compounds isorhamentin 3-O-β-D-glucoside (2), and isorhamentin 3-O-β-D-rutinoside (3) were first identified in S. glaucus. Compound 1 exhibited potent antimicrobial activities against two Gram-positive bacteria, one Gram-negative bacteria, and two fungi. Also, it displayed potent cytotoxic activity against PANC-1 cancer cell lines under glucose deficient medium (IC50 7.5 μM). However, the isolated flavonoid glycosides (2 & 3) showed moderate antimicrobial activities against two Gram-positive bacteria, two Gram-negative bacteria, four fungi, and did not show any cytotoxic activity against PANC-1.

摘要

Senecio glaucus L.干燥地上部分的乙酸乙酯部分对某些选定的细菌和真菌表现出显着的抗菌活性。此外，它在葡萄糖缺乏培养基下显示出对 PANC-1 癌细胞系的有效细胞毒性。乙酸乙酯部分经过不同的色谱技术分离生物活性化合物。一种新的苯并呋喃葡萄糖苷；分离出2,3-二氢-3β-羟基肝素3-O-吡喃葡萄糖苷( 1 )。此外，两种已知的类黄酮化合物异鼠李素 3-O- β -D-葡萄糖苷 ( 2 ) 和异鼠李糖苷 3-O- β -D-芸香苷 ( 3 ) 首次在S. glaucus中发现. 化合物1对两种革兰氏阳性菌、一种革兰氏阴性菌和两种真菌表现出有效的抗菌活性。此外，它在葡萄糖缺乏培养基 (IC50 7.5 μM) 下对 PANC-1 癌细胞系显示出有效的细胞毒活性。然而，分离的黄酮苷（2和3）对两种革兰氏阳性菌、两种革兰氏阴性菌、四种真菌显示出中等的抗菌活性，并且对 PANC-1 没有任何细胞毒活性。