ABSTRACT

Oropharyngeal Candidiasis (OPC) is an opportunistic fungal infection occurring in immunocompromised patients such as HIV/AIDS. The purpose of this study was to evaluate the antifungal properties of Lactobacillus acidophilus and Lactobacillus plantarum on different Candida species isolated from oral cavity of HIV/AIDS patients compared to Fluconazole (FLC). In this study, the antifungal effects of both cells and cell-free supernatants (CFSs) of L. acidophilus and L. plantarum were investigated against different oral Candida species by co-aggregation, agar overlay interference and broth microdilution assays, respectively. Our results showed that the highest co-aggregation ratio of the two tested Lactic acid bacteria (LAB) was observed for C. krusei. Both L. acidophilus and L. plantarum at cell concentrations 10¹⁰ to 10² cfu/ml were able to inhibit the growth of most of the oral Candida species, except for C. albicans, and to some C. krusei. In this study, MIC and MFC values for CFS of L. acidophilus ranged from 100 to 200 µl/ml and 100 to 200 µl/ml, respectively, and MIC and MFC values for CFS of L. plantarum were 50 to 200 µl/ml and 50 to 200 µl/ml, respectively. The ranges of MIC and MFC for FLC were 256–1024 µg/ml and 512–2048 µg/ml, respectively. C. albicans and C. parapsilosis displayed the highest and least susceptibility to CFSs of two LAB, respectively. Our findings showed that both cells and CFSs of L. acidophilus and L. plantarum had antifungal effects against oral Candida species.

摘要

口咽念珠菌病（OPC）是一种机会性真菌感染，发生于免疫功能低下的患者，如HIV / AIDS。这项研究的目的是评估嗜酸乳杆菌和植物乳杆菌对从艾滋病毒/艾滋病患者的口腔中分离出的不同念珠菌与氟康唑（FLC）的抗真菌特性。在这项研究中，研究了嗜酸乳杆菌和植物乳杆菌的细胞和无细胞上清液（CFS）的抗真菌作用对不同的口腔念珠菌分别通过共聚集，琼脂覆盖干扰和肉汤微稀释法进行检测。我们的结果表明，在克鲁斯克鲁维酵母中观察到两种被测乳酸菌（LAB）的最高共聚集率。既嗜酸乳杆菌和植物乳杆菌在细胞浓度10 ¹⁰至10 ² CFU / ml的能够抑制大多数口服生长念珠菌物种，除白色念珠菌，并在一定克柔念珠菌。在这项研究中，嗜酸乳杆菌的CFS的MIC和MFC值分别为100至200 µl / ml和100至200μl/ ml，而植物乳杆菌的CFS的MIC和MFC值分别为50至200 µl / ml和50至200 µl / ml。FLC的MIC和MFC范围分别为256–1024 µg / ml和512–2048 µg / ml。白色念珠菌和近平滑念珠菌分别表现出最高和最低的易感性氯氟烃2个LAB的。我们的发现表明，嗜酸乳杆菌和植物乳杆菌的细胞和CFS均对口腔念珠菌具有抗真菌作用。