the aim of this study was to test whether a single testicular needle biopsy could provide histological results comparable to en bloc resection histology and whether one biopsy was sufficient to reflect the histology of an entire pair of testicles. Two methods of sample collection were tested on 32 bull calves aged five to eight months to compare histological parameters of needle biopsy with those of en bloc resection samples. One testicular needle biopsy of the right and three en bloc samples of both testicles were collected and compared for the number of tubular cross sections, tubules with elongated spermatids (ES), outer/inner diameter of tubules, thickness of tubular wall, and number of Sertoli cells (SC). Additionally, animal data were considered. No significant differences were found between the left and right testis or among the individual locations of en bloc samples. However, histologically significant differences (Bonferroni-adjusted significance level: p < 0.05/6 = 0.0083) were found between the needle biopsy and en bloc resection regarding the tubular cross sections per visual field (p < 0.05), the outer (p = 0.01) and inner diameter and the thickness of the tubular wall (both p < 0.01). In the SOX9 immunohistochemical staining, no significant differences (p > 0.05) could be observed for SC numbers between needle biopsy and en bloc samples. In conclusion, results of testicular needle biopsy do not have the same validity as the en bloc resection histology. Furthermore, one biopsy is insufficient to reflect the histology of the entire testicular pair.

中文翻译：

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屠宰犊牛睾丸穿刺活检和整块样本的组织学比较。

这项研究的目的是检验单次睾丸穿刺活检能否提供与整体切除组织学相当的组织学结果，以及一次活检是否足以反映整对睾丸的组织学。在32头5至8个月大的牛犊上测试了两种样本采集方法，以比较穿刺活检和整块切除样本的组织学参数。收集一次右侧的睾丸穿刺活检样本和两个睾丸的三个整体样本，并比较肾小管的横截面数目，具有精子的小管（ES），肾小管的内/外直径，肾小管的壁厚和数目。支持细胞（SC）。另外，考虑了动物数据。在左右睾丸之间或整个样本的各个位置之间没有发现显着差异。但是，组织学上的显着差异（经Bonferroni调整的显着性水平：在针头活检和整块切除之间发现关于每个视野的管状横截面（p < 0.05），外部（p = 0.01）以及内部直径和管状壁厚度（p < 0.05 / 6 = 0.0083）。均p < 0.01）。在SOX9免疫组织化学染色中，针刺活检和整批样本之间的SC值未见明显差异（p> 0.05）。总之，睾丸穿刺活检的结果与整体切除组织学的有效性不同。此外，一次活检不足以反映整个睾丸对的组织学。