The ability to adapt to stress is an essential defensive function of a living body, and disturbance of this ability in the brain may contribute to the development of affective illness including major depression and anxiety disorders. A growing body of evidence suggests that brain serotonin (5-HT)_1A receptors may be involved, at least in part, in the development of adaptation to stress. 5-HT_1A receptor was reported to be transported by KIF13A, a motor protein and a member of the kinesin superfamily, from the golgi apparatus to the plasma membrane. The aim of the present study was to characterize the expression pattern of 5-HT_1A receptor and KIF13A in the hippocampus of stress-adaptive and -maladaptive mice. Mice were either exposed to repeated adaptable (1 h/day) or unadaptable (4 h/day) restraint stress, or left in their home cage for 14 days. The levels of 5-HT_1A receptor and KIF13A expression were assessed by western blot analysis. To confirm the formation of a 5-HT_1A receptor and KIF13A complex, we performed blue native-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (BN-SDS-PAGE). Western blotting showed that neither 5-HT_1A receptor nor KIF13A expression changed significantly in the hippocampal total extract of stress-adaptive and -maladaptive mice. In contrast, expression of 5 H T_1A receptor and KIF13A in the hippocampal membrane fraction was increased in stress-adaptive mice, but not in stress-maladaptive mice. BN-SDS-PAGE analysis revealed that the bands of 5-HT_1A receptor and KIF13A were both observed at a molecular weight of approximately 70 kDa, which indicated that 5-HT_1A receptor and KIF13A form a complex. The present findings suggest that translocation of 5-HT_1A receptor in complex with KIF13A to the plasma membrane of the hippocampus may play an important role in the formation of stress adaptation.

适应压力的能力是生物体的基本防御功能，大脑中这种能力的紊乱可能导致情感疾病的发展，包括重度抑郁症和焦虑症。越来越多的证据表明，脑血清素（5-HT）_1A受体可能至少部分参与了压力适应的发展。据报道5-HT _1A受体是由KIF13A，一种运动蛋白和一种驱动蛋白超家族成员从高尔基体转运到质膜的。本研究的目的是表征5-HT _1A的表达模式应激和不良适应小鼠海马中的受体和KIF13A。将小鼠暴露于反复适应性（1 h /天）或不适应性（4 h /天）的束缚压力下，或在其笼中放置14天。通过蛋白质印迹分析评估5-HT _1A受体和KIF13A表达的水平。为了确认5-HT _1A受体和KIF13A复合物的形成，我们进行了蓝色天然十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（BN-SDS-PAGE）。蛋白质印迹显示，在应激适应和适应不良小鼠的海马总提取物中，5-HT _1A受体和KIF13A表达均没有显着变化。相反，表达5 H T _1A在应激适应小鼠中，海马膜部分中的受体和KIF13A增加，而在应激适应小鼠中则没有。BN-SDS-PAGE分析表明，在约70kDa的分子量下均观察到5-HT _1A受体和KIF13A的条带，这表明5-HT _1A受体和KIF13A形成复合物。目前的发现表明5-HT _1A受体与KIF13A复合体向海马质膜的转运可能在应激适应的形成中起重要作用。