Fucoxanthin, as a main marine carotenoid, exhibit a wide variety of bioactivities, including antioxidant activity. Previously, we have shown the geroprotective activity of fucoxanthin on Drosophila and C. elegans. Our new study aimed to compare the antioxidant activity of fucoxanthin in early and late passage normal human cells LECh4(81) in physiological conditions and under oxidative stress. In addition, using the RNA-seq we have analyzed the transcriptomic changes during the replicative senescence of fibroblasts treated with fucoxanthin. Results showed that fucoxanthin at a concentration of 5 μM caused the most pronounced antioxidant effect in the late passage cells. Moreover, transcriptomic data showed the increased expression levels of genes related to the Nrf2/ARE pathway. According to the analysis of enriched KEGG pathways, fucoxanthin altered cellular processes like ribosome biogenesis, lipid metabolism, and cell cycle regulation including some age-related pathways such as Wnt, JAK-STAT, and FoxO signaling pathways. We suggest that fucoxanthin may have therapeutic potential for treating age-related diseases.

岩藻黄质作为一种主要的海洋类胡萝卜素，具有多种生物活性，包括抗氧化活性。以前，我们已经显示了岩藻黄质对果蝇和秀丽隐杆线虫的保护作用。。我们的新研究旨在比较岩藻黄质在生理条件下和氧化应激下在早期和晚期传代的正常人细胞LECh4（81）中的抗氧化活性。另外，使用RNA-seq，我们分析了用岩藻黄质处理的成纤维细胞复制衰老过程中的转录组变化。结果显示浓度为5μM的岩藻黄质在晚期传代细胞中引起最明显的抗氧化作用。此外，转录组数据显示与Nrf2 / ARE途径相关的基因表达水平增加。根据对丰富的KEGG途径的分析，岩藻黄质改变了细胞过程，如核糖体的生物发生，脂质代谢和细胞周期调控，包括一些与年龄有关的途径，如Wnt，JAK-STAT和FoxO信号传导途径。