Astyanax lacustris is a freshwater characid fish species that inhabits small streams, lakes and rivers in South America. These fish are abundant in the wild and highly adaptable in captive, being considered a good model for ecotoxicological studies. Nevertheless, there are only shortcoming gene sequence information available in public databases, which hinder their use in more comprehensive investigations that employ sensitivity molecular biology techniques to assess gene expression profile for biomarker identification. In this study, we report the first de novo transcriptome of A. lacustris liver with the aim of improving gene sequence data available for this fish species. Illumina sequencing generated 79,102,610 raw reads, which were filtered in 62,041,259 high-quality transcripts. De novo assembly resulted in 93,888 unigenes and 120,674 isoforms of an average length of 909.12 and 1046.50 bp, respectively. 60,495 isoforms (50.13%) were functionally annotated against seven databases, retrieving homology queries for about 46% of all isoforms. Therefore, in this study we provide information of relevant genes associated to environmental stress and contamination of A. lacustris, enabling future ecotoxicological researches and other molecular studies using this fish species as model.

Astyanax lacustris是一种淡水的酸性鱼类，栖息在南美的小溪，湖泊和河流中。这些鱼在野生环境中丰富，在圈养中适应性强，被认为是生态毒理学研究的良好模型。然而，公共数据库中仅有不足的基因序列信息，这阻碍了它们在更广泛的研究中的应用，这些研究采用敏感性分子生物学技术评估基因表达谱以进行生物标记鉴定。在这项研究中，我们报告了A. lacustris的第一个从头转录组肝脏，目的是​​改善可用于该鱼类的基因序列数据。Illumina测序产生了79,102,610个原始读数，并在62,041,259个高质量的转录本中进行了过滤。从头组装产生了93,888个单基因和120,674个同工型，平均长度分别为909.12和1046.50 bp。针对七个数据库在功能上注释了60,495个同工型（50.13％），检索了所有同工型中约46％的同源性查询。因此，在这项研究中，我们提供了与环境压力和湖曲霉污染相关的相关基因的信息，从而使将来以该鱼类物种为模型的生态毒理学研究和其他分子研究成为可能。