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Phosphine resistance and antioxidant enzyme activity in Trogoderma granarium Everts
Journal of Stored Products Research ( IF 2.7 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.jspr.2020.101636
Sunil Kumar Yadav , Chitra Srivastava , Subramanian Sabtharishi

Abstract In recent study, phosphine resistance was assessed in 14 populations of Trogoderma granarium collected from North India. These populations were resistant to phosphine as compared to susceptible laboratory population and the resistance ratios at LC50 ranged from 1.16 to 31.62-fold. In order to determine the trend and magnitude of evolution of phosphine resistance under laboratory conditions, phosphine resistant strain of T. granarium was developed in the laboratory by subjecting the parental strain to a selection pressure of 70% in each generation for ten successive generations. Resistant strain showed 12.74-fold resistance as compared to parental strain. Specific activity of antioxidant enzymes viz., catalase, peroxidase and superoxide dismutase was measured in both collected populations and successive generations, and correlated with phosphine resistance. Specific activity of peroxidase and superoxide dismutase was increased significantly with an increase in phosphine resistance (P

中文翻译:

Trogoderma granarium Everts 的磷化氢抗性和抗氧化酶活性

摘要 在最近的研究中,对从印度北部采集的 14 个谷斑斑病菌种群的磷化氢抗性进行了评估。与易感实验室群体相比,这些群体对磷化氢具有抗性,并且 LC50 的抗性比率范围为 1.16 至 31.62 倍。为了确定实验室条件下磷化氢抗性进化的趋势和幅度,在实验室中通过对亲本菌株进行 70% 的选择压力,连续 10 代在实验室中开发了 T. granarium 抗磷化氢菌株。与亲本菌株相比,抗性菌株显示出 12.74 倍的抗性。在收集的种群和连续世代中测量抗氧化酶的比活性,即过氧化氢酶、过氧化物酶和超氧化物歧化酶,并与磷化氢抗性相关。过氧化物酶和超氧化物歧化酶的比活性随着磷化氢抗性的增加而显着增加(P
更新日期:2020-05-01
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