Abstract In this study, peripherally and non-peripherally tetra substituted water soluble zinc(II) phthalocyanines (5a and 5b) were synthesized and characterized. Their photochemical and ct-DNA binding properties of them were investigated by UV–Vis spectroscopy. DNA cleavage effects of 5a and 5b were examined using supercoiled pBR322 plasmid DNA on agarose gel electrophoresis. In photochemical studies, the singlet oxygen quantum yields of 5a and 5b were found as 0.76 and 0.78, respectively and the photostability ratios of 5a and 5b were found as 82.12% and 85.28%. The Kb values of 5a and 5b were calculated as 2.92 ± (0.20) × 105 and 1.30 ± (0.16) × 106 M−1, respectively. In addition, 5a and 5b had low cleavage effect in dark but they showed significant photocleavage effect against plasmid DNA under light irradiation in the absence and presence of H2O2. The cytotoxic and phototoxic effects of 5b against A549 and HCT-116 cell lines were investigated using MTT cell viability assay. 5b showed significant phototoxic effects at 1, 5 and 10 μM concentrations and under irradiation on A549 and HCT-116 cell lines. These results showed that compound 5b could be used as a photosensitizer agent in PDT applications.

中文翻译：

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新型 Zn(II) 酞菁：合成、表征、光化学、DNA 相互作用和细胞毒性/光毒性

摘要 在本研究中，合成并表征了外围和非外围四取代的水溶性锌（II）酞菁（5a 和 5b）。通过紫外-可见光谱研究了它们的光化学和 ct-DNA 结合特性。使用超螺旋 pBR322 质粒 DNA 在琼脂糖凝胶电泳上检查 5a 和 5b 的 DNA 切割效果。在光化学研究中，5a和5b的单线态氧量子产率分别为0.76和0.78，5a和5b的光稳定性比分别为82.12%和85.28%。5a 和 5b 的 Kb 值分别计算为 2.92 ± (0.20) × 105 和 1.30 ± (0.16) × 106 M-1。此外，5a 和 5b 在黑暗中具有低切割效果，但在不存在和存在 H2O2 的光照射下，它们对质粒 DNA 显示出显着的光切割效应。使用 MTT 细胞活力测定研究了 5b 对 A549 和 HCT-116 细胞系的细胞毒性和光毒性作用。图 5b 在 1、5 和 10 μM 浓度下和照射下对 A549 和 HCT-116 细胞系显示出显着的光毒性作用。这些结果表明化合物5b可用作PDT应用中的光敏剂。