The biological effects of non-ionizing electromagnetic radiation (EMF) are of high importance in biological and health studies. This study aimed to find out these effects on the structure and function of recombinant human growth hormone (rhGH). The rhGH was exposed to uniform mobile phone 940-MHz EMFs for 45 min and then characterized by circular dichroism (CD), dynamic light scattering (DLS), zeta potential, and intrinsic fluorescence spectroscopy techniques. The half-life of this protein in plasma has been measured by ELISA after exposure to the EMFs. The CD spectra indicated significant changes in the secondary structure of the hormone (two negative peaks at 208 and 222 nm). These changes in the secondary structure of the treated rhGH were found irreversible as there were no significant differences in the CD spectra of the samples measured at first day and 45 days afterwards. The DLS analysis showed about 27% increase in the protein size compared to that of control. The biological activity as measured by half-life of the rhGH in plasma showed a sevenfold decrease after 25 min. The injection of the radiated protein into mice had shown a significant decrease in the weight gain compared to that of control. These findings approved irreversible disruptive changes in the structure and function of the rhGH upon exposure to the EMF.

中文翻译：

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940 MHz手机电磁场对重组人生长激素的结构和半衰期的体外影响

非电离电磁辐射（EMF）的生物学效应在生物学和健康研究中非常重要。这项研究旨在找出这些对重组人生长激素（rhGH）的结构和功能的影响。rhGH暴露于均匀的940-MHz移动电话EMMF中45分钟，然后以圆二色性（CD），动态光散射（DLS），ζ电位和固有荧光光谱技术表征。暴露于EMF后，通过ELISA测定了该蛋白在血浆中的半衰期。CD光谱表明激素的二级结构发生了显着变化（在208和222 nm处出现两个负峰）。发现处理过的rhGH二级结构的这些变化是不可逆的，因为在第一天和此后45天所测样品的CD光谱没有显着差异。DLS分析显示，与对照相比，蛋白质大小增加了约27％。通过rhGH在血浆中的半衰期测量的生物活性在25分钟后显示降低了七倍。与对照组相比，将辐射蛋白注射入小鼠的体重增加明显减少。这些发现证实了暴露于EMF后rhGH的结构和功能发生不可逆的破坏性变化。通过rhGH在血浆中的半衰期测量的生物活性在25分钟后显示降低了七倍。与对照组相比，将辐射蛋白注射入小鼠的体重增加明显减少。这些发现证实了在暴露于EMF时rhGH的结构和功能不可逆转的破坏性变化。通过rhGH在血浆中的半衰期测量的生物活性在25分钟后显示降低了七倍。与对照组相比，将辐射蛋白注射入小鼠的体重增加明显减少。这些发现证实了暴露于EMF后rhGH的结构和功能发生不可逆的破坏性变化。