This study investigated the uptake and release of Cu ions in the epiphytic lichen Evernia prunastri, a species widely used to monitor trace metal pollution. A cross transplant experiment from a background area to a polluted one and then back to an unpolluted one was simulated by incubating lichen thalli with 10 and 100 µM Cu²⁺ solutions and subsequently in deionized water to induce metal release. The working hypothesis was that after the accumulation of Cu ions, a forced release brings the concentration back to the original values. Copper treatment caused a significant uptake, proportional to the concentrations in the solution. Accumulation occurred mostly extracellularly (90% after incubation with Cu 10 µM and 60% with Cu 100 µM). The subsequent induced release was evident only in samples treated with 100 µM, and was determined by the loss from intracellular compartments. Lichen vitality, expressed in terms of photosynthetic efficiency, was not affected by Cu treatment. It is concluded that while ionic uptake is a fast process, metal release requires a much longer time. In addition, it is confirmed that the cell wall is a buffer between the outer environment and the cell interior.

这项研究调查了附生的地衣Evernia prunastri（一种广泛用于监测痕量金属污染的物种）中Cu离子的吸收和释放。通过将地衣藻与10和100 µM Cu ²⁺一起孵育，模拟了从背景区域到污染区域然后再回到未污染区域的交叉移植实验。溶液，然后在去离子水中诱导金属释放。可行的假设是，在积累铜离子之后，强制释放可使浓度恢复到原始值。铜处理导致大量吸收，与溶液中的浓度成正比。积累多发生在细胞外（与Cu 10 µM孵育后占90％，与Cu 100 µM孵育后占60％）。随后的诱导释放仅在用100 µM处理的样品中明显，并由细胞内区室的损失确定。以光合效率表示的地衣活力不受铜处理的影响。结论是，尽管离子吸收是一个快速的过程，但是金属释放需要更长的时间。此外，